MUC1黏蛋白1抗体标记超顺磁纳米颗粒在胰腺癌裸鼠模型中的实验研究

发布时间：2018-01-01 16:48

本文关键词：MUC1黏蛋白1抗体标记超顺磁纳米颗粒在胰腺癌裸鼠模型中的实验研究　出处：《中国医学影像学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的制备MUC1黏蛋白1(MUC1)靶向修饰的探针超顺磁纳米颗粒(SPION),探讨其在胰腺癌移植模型中的MRI特点。材料与方法懫用化学共轭法将MUC1与SPION耦联,构建靶向探针,检测其基本物理特性,包括水和直径、表面电荷及MR信号测定;同时建立胰腺癌皮下移植瘤裸鼠模型,研究在裸鼠体内的成像效果。取移植瘤标本,采用免疫组化及蛋白质印迹法测定MUC1的表达。结果制备的分子探针颗粒大小约为63.5 nm,表面电荷约为10.2 mV,该探针溶液能显著降低MR横向弛豫时间(T2值)。在体内实验中,MUC1可以选择性地积聚在裸鼠移植瘤模型上,并能显著降低T2信号强度。结论制备的探针具有粒径小、超顺磁性等优点,并能实现与胰腺癌组织特异性结合,通过体内成像,为疾病的诊断提供早期可靠的活体影像学资料。
[Abstract]:Objective to prepare a probe modified by MUC1 mucin 1 (MUC1) targeted for the preparation of superparamagnetic nanoparticles (SPION). To study the characteristics of MRI in pancreatic cancer transplantation model, the materials and methods were used to couple MUC1 with SPION by chemical conjugation method, to construct the target probe, and to detect its basic physical properties, including water and diameter. Surface charge and Mr signal; At the same time, the nude mice model of pancreatic cancer subcutaneous transplanted tumor was established to study the imaging effect in nude mice. The expression of MUC1 was detected by immunohistochemistry and Western blotting. Results the particle size of molecular probe was about 63.5 nm and the surface charge was about 10.2 MV. The probe solution can significantly reduce Mr transverse relaxation time and T 2 value. In vivo experiments, MUC1 can selectively accumulate in nude mice transplanted tumor model. Conclusion the prepared probe has the advantages of small particle size, superparamagnetism, and can realize the specific binding with pancreatic cancer tissue and imaging in vivo. To provide early reliable imaging data for the diagnosis of disease.
【作者单位】：复旦大学附属浦东医院肝胆外科;
【基金】：上海市浦东新区科技发展基金医疗卫生项目(PKJ2015-Y33)
【分类号】：R-332;R445.2;R735.9
【正文快照】： 分子影像学是借助于靶向性的分子探针对相关组织与器官进行定性或定量分析,并将结果可视化成像的一种全新的成像方法[1-2]。超顺磁纳米颗粒(super-paramagnetic iron oxide nanoparticles,SPION)作为新型的MRI造影剂,具有超顺磁性、良好的生物相容性、较强的可塑性等优点,适合

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