MMP-2在糖尿病模型大鼠视网膜组织中的表达

发布时间：2018-01-01 19:06

本文关键词：MMP-2在糖尿病模型大鼠视网膜组织中的表达　出处：《吉林大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的:采用腹腔注射链脲佐菌素(STZ)法复制糖尿病大鼠模型,观察糖尿病模型大鼠不同时期视网膜组织中基质金属蛋白酶2(MMP-2)表达水平,阐明MMP-2在糖尿病大鼠糖尿病视网膜病变(DR)中的作用。方法:雌性SD大鼠分为正常对照组(n=24)、4周模型组(n=30)、6周模型组(n=30)和8周模型组(n=30),模型组大鼠连续5d腹腔注射小剂量STZ复制糖尿病大鼠模型,正常对照组大鼠注射等量的柠檬酸钠溶液。4、6和8周时检测各组大鼠体质量和血糖水平。末次检测后麻醉获取大鼠右眼视网膜组织,采用RT-PCR和Western blotting法检测MMP-2mRNA及蛋白表达水平,免疫组织化学染色观察大鼠视网膜组织中MMP-2表达情况。结果:造模前,各组大鼠体质量和禁食12h的空腹血糖值比较差异均无统计学意义(P0.05)。4周模型组大鼠死亡3只,6周时死亡5只,8周时死亡7只,存活动物继续进行实验。各模型组大鼠体质量均明显低于同时期正常对照组(P0.05或P0.01),而空腹血糖值明显升高(P0.05或P0.01)。各模型组大鼠视网膜组织中MMP-2mRNA和蛋白表达水平均明显高于正常对照组(P0.05)。正常对照组大鼠视网膜结构层次清晰分明、细胞排列整齐,可见单层排列的神经节细胞,核圆形或椭圆形状,核较大;与正常对照组比较,模型组大鼠视网膜组织结构松散,内核层及视杆细胞层细胞外界膜模糊,数量减少。MMP-2阳性细胞可见棕黄色颗粒沉淀,在神经节细胞层胞浆和血管内皮细胞尤为集中。4、6及8周模型组MMP-2阳性表达水平均高于正常对照组(P0.05)。结论:MMP-2在糖尿病模型大鼠视网膜上表达,并随着糖尿病大鼠模型维持时间的延长呈递增趋势,说明MMP-2与DR有关。
[Abstract]:Objective: by intraperitoneal injection of streptozotocin (STZ) diabetic rat model was established to observe matrix metalloproteinases in retinal tissue of diabetic rats in different periods in 2 (MMP-2) expression level, clarify the MMP-2 in diabetic rats with diabetic retinopathy (DR) in rats. Methods: female SD rats were divided into the normal control group (n=24), 4 week model group (n=30), model group (n=30) for 6 weeks and 8 weeks model group (n=30), model group rats 5D continuous intraperitoneal injection of small dose STZ model of diabetic rats, normal control of sodium citrate solution in.4,6 rats injected and 8 weeks when the rats were measured body weight and blood glucose level. At the time of detection after anesthesia for the right eye retina of rats, using RT-PCR and Western blotting method to detect the expression of MMP-2mRNA and protein expression of MMP-2 in retinal tissues of rats with immunohistochemical staining: Before modeling, the body weight and fasting 12h rats fasting glucose showed no significant difference (P0.05).4 weeks rats 3 rats died in 6 weeks, 5 rats died in 8 weeks, 7 died and the survival of the animal to continue the experiment. The body weight of rats in model group were significantly at the same time lower than the normal control group (P0.05 or P0.01), and fasting blood glucose increased significantly (P0.05 or P0.01). The level was significantly higher than that of normal control group and the expression of MMP-2mRNA protein in retinal tissue of rats in the model group (P0.05). Normal control group rat retinal structure level clearly, cells arranged in neat, visible monolayer ganglion cells, nucleus round or oval shape, a large nucleus; compared with normal control group, the loose structure of retinal tissues of rats in the model group, the kernel layer and rod cell layer cell membrane outside fuzzy, reduce the number of.MMP-2 positive cells showed Brown particles sink The lake, in the ganglion cell layer of cytoplasm and endothelial cells is particularly concentrated.4,6 and 8 weeks model group MMP-2 expression levels were higher than normal control group (P0.05). Conclusion: the expression of MMP-2 in retina of diabetic rats, and with the model of diabetic rats maintain time increasing, MMP-2 and DR.

【作者单位】：承德医学院附属医院眼科;河北省秦皇岛市青龙满族自治县中医院脾肺肾科;
【基金】：河北省科技厅自然科学基金资助课题(H2015406054)
【分类号】：R-332;R587.2;R774.1
【正文快照】： 糖尿病视网膜病变(diabetic retinopahy,DR)是造成糖尿病患者视力下降的重要原因,严重者可导致失明[1]。糖尿病患者由于体内血糖水平升高,引起全身各器官各组织的微小血管出现病理改变,微血管及周围细胞出现进程性坏死或导致患者血管内皮变薄,进而导致患者体内各种屏障功能受

【相似文献】