针灸对脑性瘫痪模型幼鼠脑组织中氨基酸和神经递质的影响

发布时间：2018-01-02 01:33

本文关键词：针灸对脑性瘫痪模型幼鼠脑组织中氨基酸和神经递质的影响　出处：《中国组织工程研究》2016年40期 　论文类型：期刊论文

【摘要】：背景:针灸对脑性瘫痪幼鼠有一定的治疗作用。目的:探讨火针对脑性瘫痪幼鼠脑组织中氨基酸和神经递质表达的影响。方法:取1周龄Wistar幼鼠50只,45只结扎左侧颈总动脉建立幼鼠脑性瘫痪模型,5只不处理作为对照组。45只模型幼鼠随机分为模型组、针刺组及阳性组,每组15只。于造模第3天开始,阳性组灌胃脑酶水解片溶液,火针组使用火针处理内关穴、涌泉穴、曲池穴、百会穴,治疗持续21 d。于末次治疗1 h后,进行悬挂实验及Y型迷宫实验,并处死大鼠分离其大脑皮质及海马区,组织匀浆后检测乙酰胆碱、去甲肾上腺素、5-羟色胺、谷氨酸和γ-氨基丁酸的表达变化,采用病理切片观察海马区组织结构变化。结果与结论:(1)一般情况:21 d内治疗组大鼠活动明显多于模型组。治疗21 d,与对照组相比,模型组体质量显著下降(P0.05);与模型组相比,阳性组及火针组体质量均明显上升(P0.05),且后2组差异无显著性意义;(2)悬挂实验及Y型迷宫实验:与对照组相比,模型组悬挂时间显著下降,犯错次数显著性上升(P0.05);与模型组相比,阳性组及火针组悬挂时间显著性延长,阳性组和火针组的犯错次数显著下降(P0.05);(3)神经递质水平:与对照组相比,模型组去甲肾上腺素、5-羟色胺和γ-氨基丁酸水平下降,谷氨酸水平增加(P0.05);与模型组相比,阳性组和火针组的去甲肾上腺素、5-羟色胺和γ-氨基丁酸水平增加,谷氨酸水平减少(P0.05);(4)苏木精-伊红染色:模型组可见大量的神经元结构破坏变形,体积减小,细胞核固缩,且与细胞膜边界不清晰;阳性组和火针组都可以在一定程度上治疗脑缺血引起的海马区脑细胞结构异常。(5)结果证明,火针法可以有效治疗幼鼠脑性瘫痪。
[Abstract]:Background: acupuncture has a certain therapeutic effect on cerebral palsy young rats. Objective: to investigate the effect of fire on the expression of amino acids and neurotransmitters in brain tissue of cerebral palsy young rats. Fifty one-week-old Wistar rats were selected. 45 young rats with cerebral palsy were randomly divided into three groups: model group, acupuncture group and positive group. 15 rats in each group were treated with Neiguan points, Yongquan points, Quchi points and Baihui points in the positive group after the 3rd day of model making. After the last treatment for 1 hour, suspension test and Y-maze test were carried out. The rats were killed to isolate their cerebral cortex and hippocampus, and acetylcholine and norepinephrine were detected after tissue homogenate. Changes in the expression of 5-hydroxytryptamine, glutamate and 纬 -aminobutyric acid. Results and conclusion the activity of rats in the treatment group was significantly higher than that in the model group within 21 days after treatment, and was compared with that in the control group. The body mass of the model group decreased significantly (P 0.05). Compared with the model group, the body weight of the positive group and the fire needle group were significantly higher than that of the model group, and there was no significant difference between the latter two groups. (2) suspension test and Y-maze test: compared with the control group, the suspension time of the model group decreased significantly, and the number of mistakes increased significantly (P 0.05). Compared with the model group, the suspension time of the positive group and the fire needle group was significantly prolonged, and the number of mistakes in the positive group and the fire needle group decreased significantly (P 0.05). (3) level of neurotransmitters: compared with the control group, the levels of norepinephrine 5-hydroxytryptamine and 纬 -aminobutyric acid in the model group decreased, while the level of glutamate increased (P 0.05). Compared with the model group, the levels of norepinephrine 5-hydroxytryptamine and 纬 -aminobutyric acid in the positive group and the fire needle group were increased, and the level of glutamate was decreased (P 0.05). (4) hematoxylin-eosin staining: in the model group, a large number of neuronal structures were destroyed and deformed, the volume decreased, the nucleus was pyknosis, and the boundary with the cell membrane was not clear. Both the positive group and the fire acupuncture group could treat the abnormal structure of hippocampal cells caused by cerebral ischemia to a certain extent. The results showed that the fire acupuncture method could effectively treat cerebral palsy in young rats.
【作者单位】：河南中医学院第二附属医院小儿脑病康复科;
【基金】：河南中医学院苗圃工程项目(MP2011-16)~~
【分类号】：R245;R-332
【正文快照】： 文章快速阅读: 火针治疗后脑瘫模型幼鼠脑组织中氨基酸和神经递质的含量变化行为学检测:悬吊实验斜坡实验Y型迷宫实验 5只对照组 60只Wistar15只模型组不作处理幼鼠脑部指标检测:检测乙酰胆碱、 4脉5结只扎幼建鼠立左脑侧瘫颈模总型动1155只只阳火性针组组火脑灌针酶

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