冻融牛始基卵泡在体外三维培养的最适藻酸盐浓度

发布时间：2018-01-02 02:20

本文关键词：冻融牛始基卵泡在体外三维培养的最适藻酸盐浓度　出处：《中华生殖与避孕杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨冻融的牛始基卵泡在体外三维培养的最适藻酸钠浓度。方法玻璃化冷冻牛卵巢组织后复苏,加入Liberase DH酶消化,然后随机挑取始基卵泡,将其分别培养在二维的培养系统中(对照组,A组)和浓度分别为0.5%(B组)、1%(C组)、2%(D组)的藻酸钠三维培养系统中培养8 d,每隔1 d观察卵泡的生长情况,测量卵泡直径以及雌二醇(E_2)的分泌。结果随着培养天数增加,对照组中卵泡的完整性丧失,卵母细胞和颗粒细胞分离,而三维培养组中卵泡仍维持其完整性。体外培养8 d后,D组的卵泡平均直径为(57.7±8.5)μm,大于B组和C组(P0.05);荧光染色后,D组的未受损卵泡率为68.4%,较B组、C组高,但仅B组、D组间有统计学差异(P0.05);B组、C组、D组的E_2水平均随培养时间的延长而分泌增加,其中D组中的E_2水平(98.5±7.8)pg/mL最高,较B组、C组高,但仅B组、D组间有统计学差异(P0.05)。结论藻酸钠三维培养系统较二维培养系统更适合始基卵泡的体外培养,体积浓度为2%的藻酸钠凝胶较低浓度的藻酸钠凝胶更适合始基卵泡的生长发育。
[Abstract]:Objective to investigate the optimal concentration of sodium alginate in the freeze-thawed bovine primordial follicles in vitro. Methods the bovine ovarian tissues were vitrified and resuscitated and digested with Liberase DH enzyme. Then the primary follicles were randomly selected and cultured in a two-dimensional culture system (control group A) and the concentration of 0.5% B group. 2in group D, the follicle growth was observed every 1 day, follicular diameter and estradiol E _ 2 secretion were measured in the three-dimensional culture system of sodium alginate for 8 days. Results with the increase of culture days, the follicle diameter and the secretion of estradiol E _ 2 were measured. The integrity of follicles was lost in the control group, the oocytes and granulosa cells were separated, while the follicles in the three-dimensional culture group maintained their integrity, and the follicles were cultured in vitro for 8 days. The average diameter of follicle in group D was 57.7 卤8.5 渭 m, which was larger than that in group B and group C (P 0.05). After fluorescent staining, the rate of undamaged follicles in group D was 68.4, which was higher than that in group B and C, but only there was a significant difference between group B and group D (P 0.05). The level of E _ 2 in group B was increased with the prolongation of culture time, and the level of E _ 2 in group D was 98.5 卤7.8 g / mL, which was higher than that in group B (C). Conclusion the three-dimensional culture system of sodium alginate is more suitable for in vitro culture of primary follicles than two-dimensional culture system. Sodium alginate gel with a volume concentration of 2% is more suitable for the growth and development of primary follicles than that of low concentration alginate gel.
【作者单位】：郑州大学第三附属医院;
【分类号】：R321.1
【正文快照】： 随着癌症诊疗水平的提高,癌症患者的生存率也显著增加,但是抗癌治疗对卵巢功能存在一定的损伤,通常会破坏卵巢内始基卵泡池,从而导致急性卵巢衰竭或慢性卵巢功能不全,引起早绝经和丧失生育能力。故在癌症治疗前保存女性卵巢组织内始基卵泡变得尤为重要。而目前对始基卵泡在体

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