亚精胺对鼠免疫器官指数及炎症因子表达的影响

发布时间：2018-01-02 09:55

本文关键词：亚精胺对鼠免疫器官指数及炎症因子表达的影响　出处：《浙江农业学报》2017年02期 　论文类型：期刊论文

【摘要】：为阐明亚精胺对鼠免疫器官指数、胸腺和脾脏组织炎症因子表达的影响,采用0、0.05、0.10和0.15mg·g-1(体质量)亚精胺腹腔注射6周龄昆明小白鼠,测定鼠胸腺和脾脏指数,并定量检测胸腺和脾脏组织TNF-α、IL-1β、IL-6和IFN-γ表达量。结果表明:注射0.15 mg·g-1亚精胺组鼠胸腺指数和脾脏指数显著(P0.05)低于对照组;0.10 mg·g-1亚精胺组胸腺组织IFN-γ表达量显著(P0.05)高于对照组;0.05 mg·g-1亚精胺组鼠脾脏组织TNF-α和IL-1β表达量显著(P0.05)低于对照组,0.10 mg·g-1亚精胺组脾脏组织IL-6和IFN-γ表达量均显著(P0.05)高于对照组,0.15 mg·g-1组脾脏组织TNF-α表达量显著(P0.05)低于对照组。由此可见,亚精胺对炎症因子表达具有组织特异性和剂量依赖性。低浓度亚精胺能抑制炎症因子表达发挥抗炎作用,随着亚精胺浓度增加则表现为诱导炎症。
[Abstract]:In order to elucidate the effect of spermidine on the immune organ index and the expression of inflammatory factors in thymus and spleen of rats, 0 / 0.05 was used. 0.10 and 0.15 mg 路g-1 (body mass) spermidine were injected intraperitoneally into 6-week-old Kunming mice. The thymus and spleen indexes were measured, and TNF- 伪 in thymus and spleen tissue were measured quantitatively. The results showed that the thymus index and spleen index of mice injected with 0.15 mg 路g -1 spermidine were significantly lower than those of control group (P 0.05). The expression of IFN- 纬 in thymus of 0.10 mg 路g -1 spermidine group was significantly higher than that of control group (P 0.05). The expression of TNF- 伪 and IL-1 尾 in spleen of rats in 0.05 mg 路g -1 spermidine group was significantly lower than that in control group (P 0.05). The expression of IL-6 and IFN- 纬 in spleen of 0.10 mg 路g -1 spermidine group was significantly higher than that of control group (P 0.05). The expression of TNF- 伪 in spleen of 0.15 mg 路g -1 group was significantly lower than that of control group (P 0.05). Spermidine can inhibit the expression of inflammatory factors in a dose-dependent and tissue-specific manner, and the low concentration of spermidine can inhibit the expression of inflammatory factors, and with the increase of spermidine concentration, it can induce inflammation.
【作者单位】：四川农业大学动物科技学院/畜禽遗传资源发掘与创新利用四川省重点实验室;
【基金】：国家自然科学基金资助项目(31201798) 四川省教育综合改革试点项目“构建服务现代农业发展的产学研协同创新体系”
【分类号】：R363
【正文快照】： 多胺主要包括腐胺、亚精胺和精胺,在细胞生长、衰老、自噬和神经退行性疾病中扮演重要角色[1-2]。作为内源性的免疫调节分子,多胺与炎症密切相关,炎症组织中内源性多胺水平显著升高表明多胺在炎症反应中发挥直接作用[3]。创面坏死组织中大量产生的腐胺和尸胺经吸收入血后会显

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