siRNA干扰p38α上调人血管eNOS基因启动子转录活性

发布时间：2018-01-02 19:10

本文关键词：siRNA干扰p38α上调人血管eNOS基因启动子转录活性　出处：《暨南大学学报(自然科学与医学版)》2010年06期 　论文类型：期刊论文

【摘要】：目的:通过靶向p38α的siRNA干扰人血管内皮细胞的p38α信号通路,探索其对血管内皮型一氧化氮合酶(eNOS)基因启动子转录活性的调节作用。方法:利用W estern印迹检测siRNA-p38α干扰HUVEC-12细胞后p38α蛋白的表达,确定siRNA-p38α的最佳作用时间和浓度,然后通过双荧光素酶报告基因技术检测转染siRNA-p38α后细胞裂解液的荧光强度,分析eNOS基因启动子的转录活性变化。结果:与对照组比较,siRNA-p38α干扰后p38α蛋白表达明显减弱,而相应的eNOS启动子转录活性上调,以100 nmol/L的siRNA-p38α作用48 h的效果最佳。结论:siRNA干扰p38α信号可上调人血管内皮细胞eNOS基因的转录活性。
[Abstract]:Objective: to interfere with p38 伪 signaling pathway of human vascular endothelial cells by targeting p38 伪 siRNA. Study on the effect of Endothelial nitric oxide Synthase (Enos) on Vascular Endothelial nitric oxide Synthase. Methods: W estern blot was used to detect the expression of p38 伪 protein after siRNA-p38 伪 interfered with HUVEC-12 cells. The optimal time and concentration of siRNA-p38 伪 were determined, and the fluorescence intensity of the cell lysate after transfection of siRNA-p38 伪 was detected by double luciferase reporter gene technique. Results: compared with the control group, the expression of p38 伪 protein decreased significantly after interference of siRNA-p38 伪. The transcriptional activity of the corresponding eNOS promoter was up-regulated. The effect of siRNA-p38 伪 at 100 nmol/L for 48 h was the best. Conclusion:. SiRNA interference with p38 伪 signal upregulated the transcriptional activity of eNOS gene in human vascular endothelial cells.
【作者单位】：暨南大学生命科学技术学院组织移植与免疫实验中心;
【基金】：国家自然科学基金项目(30471635,30971465) 广东省自然科学基金项目(04010451,5006033) 暨南大学“211工程”基金项目(2009)
【分类号】：R346
【正文快照】： 人血管内皮一氧化氮合酶(endothelial nitric ox-ide synthase,eNOS)是血管内皮细胞中促进一氧化氮(nitric oxide,NO)合成的关键酶,在心、血管内皮细胞中固有表达。eNOS参与血压调整、血管通透性调节、高血压、血管狭窄和阻塞等诸多重要病理过程的发生和发展[1]。多种因素可

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