体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响

发布时间：2018-01-03 07:00

本文关键词：体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响　出处：《解放军医学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨体外光化学疗法处理的调节性树突细胞对T细胞增殖的影响。方法获取人外周血单个核细胞(PBMC),利用白介素-4重组人粒细、巨噬细胞集落刺激因子诱导生成未成熟的树突细胞(im DC)。分离获取人脾脏淋巴细胞(SP),经光化学疗法(PUVA)处理后获得PUVA-SP;将im DC与PUVA-SP共培养得到体外光化学治疗性树突细胞(ecp DC);将im DC与SP共培养得到SPDC;im DC加入10ng/ml的脂多糖(LPS),培养1d后得到DC。收集4组细胞,检测其表面抗体CD11c、CD83、CD86的表达。采用混合淋巴细胞培养的方法检测吞噬了供者凋亡的脾淋巴细胞后的受者im DC对受者T细胞增殖的影响。结果经PUVA处理后细胞早期凋亡率为91.33%。表面分子CD83、CD86在ecp DC的阳性表达率为分别为22.83%±5.26%、22.06%±4.37%,与在im DC的阳性表达率(分别为15.06%±0.59%、15.19%±1.83%)相近(P0.05),但明显低于在DC的阳性表达率(分别为99.79%±0.36%、99.85%±0.19%),差异有统计学意义(P0.01)。吞噬了供者凋亡的脾淋巴细胞后的受者im DC细胞可明显抑制受者T细胞的增殖。结论体外光化学疗法诱导的凋亡脾淋巴细胞可抑制树突细胞的成熟,并可抑制受者T淋巴细胞的增殖。
[Abstract]:Objective to investigate the in vitro photodynamic therapy treatment of regulatory dendritic cells on the proliferation of T cell. Methods of obtaining human peripheral blood mononuclear cells (PBMC), using recombinant human interleukin -4 granule, macrophage colony stimulating factor induced the formation of immature dendritic cells (im DC). Isolated from human spleen lymphocytes (SP) photodynamic therapy (PUVA), was obtained after the treatment of PUVA-SP; Im DC and PUVA-SP were cultured in vitro by photochemical treatment of dendritic cells (ECP DC); Im DC co cultured with SP SPDC; Im DC 10ng/ml with lipopolysaccharide (LPS), after 1D DC. collected from 4 groups of cells, the detection of surface CD11c CD83, CD86 antibody, expression. By using the method of mixed lymphocyte culture test swallowed the effect of donor apoptotic splenocytes after recipient im DC on the proliferation of T cells. Results after treatment of PUVA cells early apoptosis rate of 91.33%. surface Molecular CD83, CD86 positive expression rate of DC in ECP were 22.83% + 5.26%, 22.06% + 4.37%, and the positive expression rate of DC in im (15.06% + 0.59%, 15.19% + 1.83%) similar to (P0.05), but was significantly lower than that in the positive expression rate of DC (99.79% + 0.36%, 99.85% + 0.19%), the difference was statistically significant (P0.01). The phagocytosis of donor spleen lymphocyte apoptosis after IM infected DC cells can significantly inhibited the proliferation of T cell apoptosis induced by splenic lymphocytes. Conclusion extracorporeal photochemotherapy can inhibit the maturation of dendritic cells, and inhibited the proliferation of T lymphocytes.

【作者单位】：解放军309医院全军器官移植研究所肝胆外科;解放军309医院器官移植与免疫调节北京市重点实验室;
【基金】：国家自然科学基金(811022422)~~
【分类号】：R392
【正文快照】： 性免疫反应[1-3]。体外光化学疗法(extracorporealphotochemotherapy,ECP)是一种新型免疫疗法,与树突细胞共同作用可诱导生成凋亡的人脾淋巴细胞(SP)[4]。本研究应用未成熟的树突细胞(immaturedendritic cells,im DC)吞噬凋亡的SP,制备了一种新型的调节性树突细胞(regulatory

【相似文献】