补肾益智提取物对AD小鼠模型脑内去甲肾上腺素及肾上腺素的调控研究

发布时间：2018-01-03 08:09

本文关键词：补肾益智提取物对AD小鼠模型脑内去甲肾上腺素及肾上腺素的调控研究　出处：《时珍国医国药》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨不同脑区去甲肾上腺素(NE)及肾上腺素(E)浓度变化与病理形态之间的关系及补肾益智方提取物对其的调控作用。方法采用东莨菪碱诱导的小鼠作为阿尔茨海默病(Alzheimer's Disease,AD)的动物模型,运用HE染色观察补肾益智提取物对AD模型小鼠脑组织病理形态影响,运用高效液相色谱-荧光检测器定量检测不同脑区NE及E含量变化。结果模型组小鼠脑组织海马和皮质出现大量的神经元坏死;与模型组比,补肾益智方提取物处理组海马和皮质的病理变化减轻。与正常组相比,模型组小鼠脑组织皮质NE和E及海马NE均升高,差异均有统计学意义(P0.01);与模型组相比,补肾益智提取物低剂量和高剂量组皮质NE和E均减少,差异有统计学意义(P0.01)。结论以补肾为靶点的益智提取物对东莨菪碱急性AD小鼠模型脑组织NE和E浓度异常具有调控作用,为阿尔茨海默病的发病机制提供了新的方向和依据。
[Abstract]:Objective to explore the different brain regions of NE) and epinephrine (E). The relationship between the change of concentration and pathological morphology and the regulation of extract of Bushen Yizhi prescription on it. Methods Scopolamine induced mice were used as Alzheimer's disease (AD). Alzheimer's Disease. He staining was used to observe the effect of the extract of tonifying kidney on brain histopathology of AD mice. The contents of NE and E in different brain regions were quantitatively detected by high performance liquid chromatography-fluorescence detector. Results A large number of neuronal necrosis occurred in hippocampus and cortex of mice in the model group. Compared with the model group, the pathological changes of hippocampus and cortex were alleviated in Bushen Yizhi extract group. Compared with normal group, NE and E in brain tissue and NE in hippocampus were increased in model group. The difference was statistically significant (P 0.01). Compared with model group, NE and E in cortex of low dose and high dose group were decreased. Conclusion the brain NE and E concentrations of scopolamine acute AD mice model can be regulated by the extract of Yizhi, which is aimed at tonifying the kidney, and the difference is significant (P 0.01). Conclusion the concentration of NE and E in brain tissue of scopolamine acute AD mice can be regulated by the extract. It provides a new direction and basis for the pathogenesis of Alzheimer's disease.
【作者单位】：泰山医学院药理研究所;广州中医药大学临床药理研究所;
【基金】：广东省重大科技专项(No.2012A080202017) 山东省政府"泰山学者海外特聘专家"专项基金泰山地产中药研发协同创新中心项目山东省自然科学基金(No.ZR2016HB56) 山东省泰安市科技局项目(No.2016NS1078)
【分类号】：R285.5;R-332
【正文快照】： 阿尔茨海默病(Alzheimer's disease,AD)又称老年性痴呆,是一种以进行性认知障碍和记忆力减退为主要临床表现的致死性神经退行性疾病。学习记忆的脑功能定位表明,记忆痕迹的贮存部位主要位于大脑皮层联络区、海马及其邻近结构、丘脑和脑干网状结构等[1]。从神经生化角度看,中枢

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