加味麻黄蝉衣汤对变应性鼻炎模型豚鼠的改善作用及其机制研究

发布时间：2018-01-03 08:28

本文关键词：加味麻黄蝉衣汤对变应性鼻炎模型豚鼠的改善作用及其机制研究　出处：《中国药房》2016年04期 　论文类型：期刊论文

【摘要】：目的:研究加味麻黄蝉衣汤对变应性鼻炎(AR)模型豚鼠的改善作用及其机制。方法:将32只豚鼠随机均分为正常(0.9%氯化钠注射液)组、模型(0.9%氯化钠注射液)组、实验[加味麻黄蝉衣汤,5.18 g(生药)/kg]组和对照[丙酸氟替卡松鼻喷雾剂,50μg/(只·d)]组,除正常组外其余各组豚鼠ip卵清蛋白和氢氧化铝各30 mg复制AR模型。除对照组喷雾给药外,其余各组ig相应药物,每天2次,连续15 d。观察豚鼠的鼻部症状并进行评分,免疫组化法检测鼻黏膜酪氨酸磷酸酶1(SHP-1)的蛋白表达水平,酶联免疫吸附法检测血清中白细胞介素4(IL-4)、干扰素γ(IFN-γ)的含量。结果:与正常组比较,模型组豚鼠出现AR症状,评分增加,鼻黏膜SHP-1蛋白表达水平升高,血清中IL-4含量增加、IFN-γ含量减少(P0.05);与模型组比较,实验组和对照组豚鼠AR症状改善,评分降低,鼻黏膜SHP-1蛋白表达水平降低,血清中IL-4含量减少、IFN-γ含量增加(P0.05)。结论:加味麻黄蝉衣汤对AR有一定的改善作用,其机制可能与减少血清中IL-4的含量、增加血清中IFN-γ的含量有关。
[Abstract]:Objective: to study the effect of modified ephedra cicada decoction on allergic rhinitis and its mechanism in guinea pigs. Methods: 32 guinea pigs were randomly divided into two groups: normal group (0.9% sodium chloride injection). Model 0. 9% sodium chloride injection) group, experiment. [Modified ephedra cicadas decoction 5.18 g (crude drug P / kg) group and control group. [Fluticasone propionate nasal spray (50 渭 g / d) was used to establish AR model in guinea pigs except the normal group (30 mg ovalbumin and 30 mg aluminum hydroxide). The other groups were given corresponding drugs twice a day for 15 days. The nasal symptoms of guinea pigs were observed and scored. The protein expression of tyrosine phosphatase 1 (SHP-1) in nasal mucosa was detected by immunohistochemistry. Results: compared with the normal group, the model group showed AR symptoms and increased the score. The expression of SHP-1 protein in nasal mucosa was increased, and the content of IL-4 in serum was increased. The content of IFN- 纬 was decreased (P 0.05). Compared with the model group, the symptoms of AR in the experimental group and the control group were improved, the score was decreased, the expression of SHP-1 protein in nasal mucosa was decreased, and the content of IL-4 in serum was decreased. The content of IFN- 纬 increased P0.050.Conclusion: modified ephedra cicadas decoction can improve AR to some extent, and its mechanism may be related to the reduction of IL-4 content in serum. Increasing the level of IFN- 纬 in serum was related to the increase of IFN- 纬 in serum.
【作者单位】：湖北民族学院附属民大医院耳鼻咽喉科;
【分类号】：R285.5;R-332
【正文快照】： *主治医师,硕士。研究方向:变应性鼻炎的诊疗及基础研究。E-mail:13687100@qq.com近年来变应性鼻炎(AR)的发病率有升高的趋势,且发病人群趋于年轻化。虽然有多种传统药物治疗以及外科手术、免疫治疗等方法已经投入到AR的治疗中[1-2],但是由于AR发病机制复杂,治疗效果均不佳。

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