尿酸排泄不良型高尿酸血症动物模型的建立

发布时间：2018-01-04 10:44

  本文关键词：尿酸排泄不良型高尿酸血症动物模型的建立　出处：《中国比较医学杂志》2017年08期 　论文类型：期刊论文

  更多相关文章： 高尿酸血症 氧嗪酸钾 吡嗪酰胺 乙胺丁醇 大鼠动物模型

【摘要】：目的探讨构建尿酸排泄不良型高尿酸血症大鼠模型的可靠方法,为尿酸排泄不良型高尿酸血症发病机制的研究及治疗方案的优化奠定基础。方法采用氧嗪酸钾(300 mg/kg)分别与吡嗪酰胺(300 mg/kg)、乙胺丁醇(250 mg/kg)联合造模。连续给药1周、3周、5周,检测大鼠血、尿、便中尿酸水平,同时检测肝、肾功能,并进行组织学病理切片观察。结果氧嗪酸钾与乙胺丁醇联合造模组大鼠血尿酸出现先升高后降低的现象,而氧嗪酸钾与吡嗪酰胺联合造模组在连续给药期间血尿酸和尿液中尿酸升高平稳。两种方法对肝脏、肾脏均无明显损害。结论氧嗪酸钾与吡嗪酰胺联合能有效建立尿酸排泄不良型高尿酸血症大鼠模型,且模型稳定性好,其发病过程更符合临床尿酸排泄不良型高尿酸血症的病程。
[Abstract]:Objective to explore the method of constructing reliable uric acid excretion adverse type hyperuricemia rat model, and lay the foundation for the optimization of treatment and pathogenesis of hyperuricemia uric acid excretion adverse type. Method using potassium oxonate (300 mg/kg) and pyrazinamide (300 mg/kg), ethambutol (250 mg/kg) combined with continuous administration model. 1 weeks, 3 weeks, 5 weeks, rats were detected in blood, urine, feces and uric acid, renal function, and detection of liver histological pathological observation. The potassium oxonate and ethambutol combined model rats serum uric acid increased first and then decreased, while the potassium oxonate and combined with pyrazinamide model group continuously during the administration of blood uric acid and urine uric acid increased steadily. The two methods on the liver and kidneys were no significant damage. Conclusion potassium oxonate combined with pyrazinamide can effectively establish uric acid excretion bad high uric acid in the blood The model of the rat, and the model is stable, is more consistent with the course of hyperuricemia in clinical uric acid excretion.

【作者单位】： 辽宁中医药大学;辽宁中医药大学附属医院;
【基金】：辽宁省卫计委资助项目(LNCCC-D54-2015) 沈阳市科技计划项目(F15-139-9-39)
【分类号】：R-332;R589.7
【正文快照】： 尿酸是嘌呤代谢的最终产物,由于人类的尿酸酶基因在进化过程中发生了突变,缺乏尿酸酶,嘌呤代谢最终大部分以尿酸形式排出,这使得人类的血尿酸水平远远高于其他哺乳动物。而实验常用的啮齿类动物,因为具有尿酸酶,可将尿酸降解为尿囊素后排出体外[1]。因此,为了使动物模型与人类，

本文编号：1378139