IL-1β和MMP-13在兔骨关节炎模型软骨和滑液中的表达

发布时间：2018-01-05 08:18

本文关键词：IL-1β和MMP-13在兔骨关节炎模型软骨和滑液中的表达　出处：《西安交通大学学报(医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的评价兔膝关节内侧半月板损伤制作骨关节炎(OA)模型的方法的可行性,探讨白细胞介素-1β(IL-1β)和金属蛋白酶-13(MMP-13)在兔骨关节炎模型软骨和滑液病变中的作用机制。方法新西兰大白兔40只,随机分为实验组(n=30)和对照组(n=10),于术后2、6、12周观察股骨髁关节软骨的病理变化并进行评分;用免疫组化方法检测关节软骨中IL-1β和MMP-13的表达情况;用酶联免疫吸附法测定关节液中IL-1和MMP-13的含量。结果实验组和对照组在大体和病理观察不同时间点关节软骨评分及HE染色差异均有统计学意义(P0.05)。免疫组织化学检测结果显示IL-1β在两组中均有表达,实验组和对照组2周时细胞分数差异无统计学意义,6、12周差异有统计学意义(P0.05)。MMP-13在对照组关节软骨细胞中未见表达,实验组的关节软骨细胞中可见MMP-13蛋白的表达,差异有统计学意义(P0.05)。关节滑液中IL-1β表达和在软骨中表达一致。结论采用损伤兔膝关节半月板的方法可建立合理的动物OA模型;IL-1β和MMP-13在OA发病过程中表达水平有明显变化,需要进一步的临床研究来探讨其是否可以作为早期诊断OA的指标之一。
[Abstract]:Objective to evaluate the feasibility of making OAA model with medial meniscus injury of knee joint in rabbits. To investigate the mechanism of interleukin-1 尾 interleukin-1 (IL-1 尾) and metalloproteinase-13 (MMP-13) in cartilage and synovial fluid of rabbit osteoarthritis model. The rats were randomly divided into two groups: the experimental group (n = 30) and the control group (n = 10). The pathological changes of the articular cartilage of the femoral condyle were observed and evaluated at 2 ~ 6 ~ 12 weeks postoperatively. The expression of IL-1 尾 and MMP-13 in articular cartilage was detected by immunohistochemistry. The contents of IL-1 and MMP-13 in articular fluid were measured by enzyme linked immunosorbent assay (Elisa). Results there were significant differences in articular cartilage score and HE staining between the experimental group and the control group at different time points of gross and pathological observation. Righteousness (. The results of immunohistochemistry showed that IL-1 尾 was expressed in both groups. There was no significant difference in cell fractions between the experimental group and the control group at the 2nd week. There was no significant difference in the expression of MMP-13 in the articular chondrocytes of the control group at 12 weeks. The expression of MMP-13 protein was observed in articular chondrocytes of experimental group. The difference was statistically significant (P 0.05). The expression of IL-1 尾 in synovial fluid was consistent with that in cartilage. Conclusion A reasonable animal model of OA can be established by using meniscus of knee joint injury in rabbits. The expression levels of IL-1 尾 and MMP-13 in the pathogenesis of OA have obvious changes, and further clinical research is needed to explore whether they can be used as one of the indicators for early diagnosis of OA.
【作者单位】：新乡医学院第一附属医院骨科;
【基金】：国家自然科学基金资助项目(No.81672190)~~
【分类号】：R-332;R684.3
【正文快照】： 骨性关节炎(ostcoarthritis,OA)是临床上常见的骨关节疾病,由于关节软骨退变的生化改变早于形态学改变,因此有必要找到一种能及时反映OA早期生化改变的生物标志物(biomarker,BM),以便临床早期发现OA,有效的诊断和治疗早期骨关节炎。目前,已发现可反映关节软骨、滑膜、骨的代谢

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