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刺苋花粉过敏性评价研究

发布时间:2018-01-06 00:17

  本文关键词:刺苋花粉过敏性评价研究 出处:《核农学报》2017年10期  论文类型:期刊论文


  更多相关文章: 刺苋花粉 超声提取 动物模型 BALB/c小鼠 HE染色


【摘要】:刺苋是一种外来入侵植物,刺苋花粉现已成为我国华南地区的重要过敏原,且刺苋花粉变应原易与其他过敏原交叉反应。为研究刺苋花粉的过敏性,以刺苋花粉为研究对象对其进行致敏原纯化,再利用BALB/c小鼠进行腹腔注射,致敏滴鼻激发建立BALB/c小鼠动物过敏评价模型。通过对小鼠鼻部症状观察及评分,鼻腔分泌物涂片染色观察和血清中小鼠组胺和Ig E含量的测定,以及对小鼠解剖后肺部HE染色观察来确定小鼠的致敏剂量。结果表明,小鼠体内Ig E含量随注射剂量的增加呈线性增长的趋势,在注射量为75μg时达到最大值;小鼠体内组胺含量在低剂量注射时呈线性增长趋势,在注射量达到38μg后组胺含量变化趋于平缓;75μg注射量可引起小鼠明显的过敏反应,且导致小鼠肺部炎症反应。本研究对刺苋花粉进行过敏性评价研究,为全面进行外来入侵植物的健康风险评价做了一定补充。
[Abstract]:Amaranthus spinosus is a kind of alien invasive plants, Amaranthus spinosus has become China's Southern China region an important allergen, and Amaranthus spinosus pollen allergen and other allergens for allergic reactions. The cross research of Amaranthus spinosus, with Amaranthus spinosus as the research object were sensitized to the original purification and reuse of BALB/c mice intraperitoneal injection, intranasal sensitization induced BALB/c mice animal allergy evaluation model. Through the observation and mouse nasal symptoms score, and serum were observed in mice of histamine and Ig content of E staining in nasal secretions, and the staining of lung in HE mice after dissection to determine the dose of sensitized mice. The results showed that the content of Ig E mice with linearly increasing dose, the injection amount of 75 g reached the maximum value; the content of histamine in mice showed a linear increasing trend in the low dose of injection, the injection amount of At 38 G after histamine content changes slowly; 75 g injection volume can cause allergic reactions in mice significantly, and cause lung inflammation in mice. The research on the evaluation of Amaranthus spinosus pollen allergy, do some supplement for health risk assessment of invasive plants comprehensively.

【作者单位】: 中国计量大学生命科学学院;浙江省海洋食品品质及危害物控制技术重点实验室;海洋食品加工质量控制技术与仪器国家地方联合工程实验室;
【基金】:质检类公益项目(201410014-5)
【分类号】:R392
【正文快照】: 花粉主要由脂质、多糖、蛋白质及小分子化合物构成,其中某些蛋白质是致敏的主要成分。花粉作为吸入性变应原进入人体后可发生I型过敏反应,主要危害呼吸道,甚至引起肺部炎症,对人体健康构成巨大威胁。在花粉激发状态下,效应细胞释放的生物活性介质与相应的受体发生特异性结合,

本文编号:1385483

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