2013年~2015年广东地区实验小鼠和大鼠微生物学及寄生虫学调查

发布时间：2018-01-06 08:23

本文关键词：2013年~2015年广东地区实验小鼠和大鼠微生物学及寄生虫学调查　出处：《中国比较医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的调查2013~2015年广东地区实验小鼠和大鼠的病原携带情况。方法本文涉及的样品主要来源于监督检测的抽样样品和委托检测的送样样品,共收集到广东省12家监督单位和32家委托单位样品。按照国家标准要求的项目及标准外的螺杆菌和小鼠诺如病毒进行检测。结果监督检测和委托检测结果存在较大差异。3年中监督检测的小鼠检出4种病原,包括绿脓杆菌(0.7%)、嗜肺巴斯德杆菌(0.3%)、肺炎克雷伯杆菌(0.7%)和鞭毛虫(1.7%),未检出病毒;大鼠检出1种病原即嗜肺巴斯德杆菌(1.1%),未检出病毒和寄生虫。委托检测的小鼠检出15种病原,包括绿脓杆菌(3.7%)、嗜肺巴斯德杆菌(5.2%)、支原体(1.9%)、金黄色葡萄球菌(0.7%)、肺炎克雷伯杆菌(0.8%)、螺杆菌(45.3%),小鼠肝炎病毒(8.5%)、小鼠脑脊髓炎病毒(7.0%)、小鼠诺如病毒(16.2%)、鼠痘病毒(0.3%)、小鼠细小病毒(0.5%)、仙台病毒(0.1%)、鞭毛虫(11.7%)、蠕虫(1.0%)、体外寄生虫(0.1%)。大鼠检出8种病原,包括绿脓杆菌(3.4%)、嗜肺巴斯德杆菌(8.6%)、支原体(0.9%)、金黄色葡萄球菌(2.9%)、泰泽病原体(4.8%)、大肠杆菌(1.0%)、大鼠细小病毒H-1株(3.0%)、大鼠冠状病毒(1.0%),未检出寄生虫。其中,实验小鼠7种病原,包括绿脓杆菌、嗜肺巴斯德杆菌、螺杆菌、鞭毛虫、小鼠肝炎病毒、小鼠脑脊髓炎病毒和小鼠诺如病毒,大鼠2种病原,包括金黄色葡萄球菌和泰泽病原体,检出范围广、检出率较高,而且存在区域分布特点,即设施污染后能在多次送检的动物中检出这些病原。结论本研究获得广东省实验大小鼠病原流行情况和分布,这些数据的统计为我国实验动物质量标准的制订提供基础数据,同时也为各动物实验设施的质量控制方案制订提供依据。
[Abstract]:Objective to investigate the pathogenicity of experimental mice and rats in Guangdong from 2003 to 2015. A total of 12 surveillance units and 32 entrustment units were collected from Guangdong Province for detection of Helicobacter pylori and norovirus in mice in accordance with the items and standards required by the national standard. Results the results of monitoring and entrusting detection were kept. Four kinds of pathogens were detected in the mice which were monitored and detected in three years. The virus was not detected, including P. aeruginosa 0.7m, Pasteurella pneumophila 0.3and Klebsiella pneumoniae 0.7m. One pathogen, Pasteurella pneumophilus, was detected in rats, and no virus or parasite was detected. Fifteen pathogens, including Pseudomonas aeruginosa, were detected in the commissioned mice. Pasteurella pneumophila 5.2%, Mycoplasma 1.9%, Staphylococcus aureus 0.7%, Klebsiella pneumoniae 0.8%, Helicobacter pneumoniae 45.35.35). Mouse hepatitis virus (RHV), mouse encephalomyelitis virus (MDV), mouse encephalomyelitis virus (mNV), mouse norovirus (RV), mouse pox virus (RV), mouse parvovirus (mPV), and mouse parvovirus (mPV). Sendai virus was 0.1 and flagellum was 11.70.The worm was 1.0 and the extracorporeal parasite was 0.1. Eight pathogens, including Pseudomonas aeruginosa, were detected in rats. Pasteurella pneumophila 8.6m, Mycoplasma 0.910, Staphylococcus aureus 2.9%, Taisei pathogen 4.810, Escherichia coli 1.0). No parasites were detected in rat parvovirus H-1 strain and rat coronavirus strain 1.0. Among them, there were 7 pathogens in experimental mice, including Pseudomonas aeruginosa, Pasteurella pneumophila and Helicobacter pneumoniae. Flagellate, mouse hepatitis virus, mouse encephalomyelitis virus and mouse norovirus, rat two kinds of pathogens, including Staphylococcus aureus and Taisei pathogen, the detection range is wide, the detection rate is high. And there are regional distribution characteristics, that is, the facility pollution can detect these pathogens in animals submitted for many times. Conclusion in this study, the epidemic situation and distribution of experimental rats and mice in Guangdong Province were obtained. The statistics of these data provide the basic data for the establishment of the quality standard of laboratory animals in China, and also provide the basis for the formulation of the quality control schemes of the animal experimental facilities.
【作者单位】：广东省实验动物监测所;广东省实验动物重点实验室;
【基金】：广东省科技计划项目(2013B060400028,2014A070705003,2014B070706006,2015A030302028)
【分类号】：R-332
【正文快照】： Guangdong province from 2013 to 2015.Methods The samples of mice and rats were collected from 12 supervised and32 entrusting institutions in Guangdong province.The test items and methods were determined according to the nationalstandards,additionally,the

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