急性冷应激大鼠肝脏中mir-210的生物学功能预测

发布时间：2018-01-06 09:21

本文关键词：急性冷应激大鼠肝脏中mir-210的生物学功能预测　出处：《生理学报》2016年02期 　论文类型：期刊论文

更多相关文章： 冷应激 mir- 肝脏 qRT-PCR 生物信息学

【摘要】：本文通过观察冷应激时mir-210在大鼠肝脏中的表达情况,预测了mir-210在冷应激中的功能。本实验采用了30只12周龄SPF级Wistar雄性大鼠,体重(340±20)g,将大鼠在常温预饲一周后,随机分成急性冷应激组[温度(4±0.1)°C]和常温对照组[温度(24±0.1)°C],急性应激12 h后,提取肝脏,q RT-PCR检测mir-210表达。结果表明,急性冷应激组大鼠肝脏中的mir-210表达量升高,与常温对照组相比差异极显著(P0.01)。通过生物信息学方法分析发现,mir-210的靶基因有上百种,其中与冷应激相关性较高的靶基因是E2F3、RAD52、ISCU和Ephrin-A3。ISCU能够调节细胞的呼吸代谢,而Ephrin-A3对细胞的增殖和凋亡有显著影响;mir-210的上调还能够直接影响DNA的修复机制,从而导致遗传不稳定。结果提示,冷应激时肝脏mir-210的上调能够对细胞生长发育、能量代谢和遗传等方面产生影响。
[Abstract]:In this paper, through the observation of cold stress on the expression of mir-210 in rat liver, predicted the mir-210 in cold stress function. This experiment used 30 SPF of 12 week old male Wistar rats, weight (340 + 20) g, the rats in normal pre feeding after one week, were randomly divided into acute cold temperature stress group [(4 + 0.1) C] and control group [temperature (24 + 0.1) C], 12 h after acute stress, extraction of liver, detect the expression of mir-210 Q RT-PCR. The results showed that acute cold stress rat liver mir-210 expression increased, compared with the normal temperature control group significant differences (P0.01). Through the method of bioinformatics analysis showed that the target gene mir-210 has hundreds of target genes, and the high correlation of cold stress is E2F3, RAD52, ISCU and Ephrin-A3.ISCU can regulate the respiratory metabolism of cells, and Ephrin-A3 on the proliferation and apoptosis of cells were significantly affected by the upregulation of mir-210; also It can directly affect the repair mechanism of DNA, which leads to genetic instability. It is suggested that the up regulation of liver mir-210 in cold stress can affect cell growth, energy metabolism and heredity.

【作者单位】：黑龙江八一农垦大学动物科技学院;
【基金】：support by the National Natural Science Foundation of China(No.31372398) Ph.D.Programs Foundation of Ministry of Education of China(No.20132305110004) the Natural Science Foundation of Heilongjiang Province of China(No.C2015041) Open Project of the Key Laboratory Subject of Heilongjiang Provincial Department of Education of China(No.AMKL201302)
【分类号】：R33
【正文快照】： This work was support by the National Natural Science Foundation of China(No.31372398),Ph.D.Programs Foundationof Ministry of Education of China(No.20132305110004),the Natural Science Foundation of Heilongjiang Province of China(No.C2015041)and Open Proj

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