匹罗卡品诱导癫痫大鼠模型小胶质细胞的激活与极化

发布时间：2018-01-06 15:36

本文关键词：匹罗卡品诱导癫痫大鼠模型小胶质细胞的激活与极化　出处：《昆明医科大学学报》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨癫痫大鼠模型发作不同时间点,小胶质细胞的激活和极化情况.方法 80只雄性SD大鼠随机分为5组:即对照组、癫痫发作后1 d、3 d、7 d、14 d模型组.采用氯化锂-匹罗卡品联合制备癫痫模型,对照组给予相同剂量的生理盐水,发作相应时间点取材,免疫荧光染色观察小胶质细胞激活的形态学变化,免疫组织化学染色检测i NOS和Arg-1的表达变化.结果癫痫发作后不同时间点均有小胶质细胞的激活,细胞突触减少,胞体变圆,大部分呈阿米巴样;与对照组相比,模型组在癫痫发作后不同时间,i NOS的表达均有升高,Arg-1的表达均下降,3 d最为明显,差异有统计学意义(P0.05).结论在匹罗卡品诱导的癫痫大鼠模型中,小胶质细胞在癫痫发作后不同时间出现明显激活和极化.
[Abstract]:Objective to investigate the activation and polarization of microglia at different time points in epileptic rat model. Methods 80 male SD rats were randomly divided into 5 groups: control group, 1 day after seizure and 3 days after seizure. The epileptic model was made with lithium-pilocarpine combined with lithium-pilocarpine for 7 days. The control group was given the same dose of normal saline, and the corresponding time points of seizure were obtained. The morphological changes of microglia activation were observed by immunofluorescence staining. Results the expression of I NOS and Arg-1 was detected by immunohistochemical staining. Results the activation of microglia, the decrease of synapse and the roundness of cell body were observed at different time points after seizure. Most of them were amoeba like. Compared with the control group, the expression of NOS in the model group increased at different time points after seizure, and the expression of Arg-1 decreased significantly at 3 days after seizure. Conclusion in the rat model of pilocarpine induced epilepsy, microglia were activated and polarized at different time after seizure.
【作者单位】：昆明医科大学第一附属医院神经内科;昆明医科大学生物医学工程研究中心;昆明医科大学人体解剖学与组织胚胎学系;昆明医科大学体育部;
【基金】：国家自然科学基金资助项目(81360200;81460210;81260297;81460350) 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2013FB120;2014FB004;2015FB005)
【分类号】：R742.1;R-332
【正文快照】： 癫痫(epilepsy,EP)是一种慢性、反复发作的以颅内神经元异常放电导致的短暂大脑功能障碍为基础的中枢神经系统疾病,该病发病机制与神经元的兴奋性及神经胶质细胞的异常改变都有密切关系.小胶质细胞(microglia,MG)是存在于中枢神经系统(central nervous system,CNS)的具有免疫

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