骨髓源干细胞促进皮肤软组织扩张新生的研究

发布时间：2018-01-06 15:12

本文关键词：骨髓源干细胞促进皮肤软组织扩张新生的研究　出处：《广西医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的皮肤软组织扩张可以刺激皮肤基底层细胞增殖,真皮胶原增多和血管新生。我们将绿色荧光蛋白(enhanced green fluorescent protein, EGFP)转基因鼠的骨髓移植到C57BL鼠体内,制作成嵌合鼠模型,以探究扩张过程中骨髓源干细胞(bone marrow-derived stem cells, BMSCs)对新生皮肤的作用。方法取绿色荧光蛋白转基因鼠股骨和胫骨的骨髓,由静尾静脉移植到C57BL鼠体内,制作成嵌合鼠。嵌合鼠被随机分为A组、B组和C组。在A组嵌合鼠背部埋置硅胶扩张器,并按时注入生理盐水；在B组嵌合鼠背部同法埋置硅胶扩张器,不注水；C组嵌合鼠不埋置扩张器。分别在术后0,7,14,21,28,35天获取扩张皮肤标本。检测皮肤中基质衍生因子(Stromal-Derived Factor-1, SDF-1)的表达量。荧光显微镜观察EGFP+细胞在扩张皮肤中的数量、分布和表型变化。结果嵌合鼠皮肤中EGFP细胞稳定存在。A组嵌合鼠皮肤中的EGFP+细胞数量随着张力刺激而变化,21天时达到最多(17.1±6.7%)；而B组和C组分别为(5.5±1.0%)和(5.1±0.9%)。同时,扩张皮肤与非扩张皮肤相比SDF-1的表达量有明显差异(约11倍,p0.01)。免疫荧光显示EGFP+细胞转化为血管内皮细胞,表皮细胞和纺锤状的真皮成纤维细胞。结论在张力作刺激下,扩张皮肤中SDF-1的表达量增多,BMSCs稳定存在扩张皮肤中,并且发生增殖和分化。
[Abstract]:Objective skin soft tissue expansion can stimulate the proliferation of skin basal layer cells. Increased dermal collagen and angiogenesis. We enhanced green fluorescent protein with green fluorescent protein. The bone marrow of EGFP transgenic mice was transplanted into C57BL mice to make chimeric mouse model. To explore the effect of bone marrow-derived stem cells (BMSCs) on newborn skin during dilatation. Methods Bone marrow from femur and tibia of transgenic mice with green fluorescent protein were transplanted into C57BL mice by tail vein, and the chimeric mice were randomly divided into A group. Group B and group C. silicone dilator was implanted on the back of chimeric rats in group A and saline was injected on time. In group B chimeric rats were implanted with silica gel expander in the same way on the back without water injection. In group C, the dilator was not embedded in the chimeric rats. The expanded skin samples were obtained for 35 days. Stromal-derived Factor-1 was detected. The number, distribution and phenotypic changes of EGFP cells in dilated skin were observed by fluorescence microscope. Results the number of EGFP cells in the skin of the chimeric mice was stable. The number of EGFP cells in the skin of the chimeric mice in group A was 17.1 卤6.7 at 21 days after stress stimulation. In group B and C, it was 5.5 卤1.0 and 5.1 卤0.9, respectively. Meanwhile, the expression of SDF-1 in dilated skin was significantly different from that in non-expanded skin (about 11 times). Immunofluorescence showed that EGFP cells were transformed into vascular endothelial cells, epidermal cells and fusiform dermal fibroblasts. Conclusion under the stimulation of Zhang Lizuo, the expression of SDF-1 in expanded skin was increased.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

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