微小RNA-21对人脐血间充质干细胞增殖和转化生长因子-β1表达的影响

发布时间：2018-01-06 18:26

本文关键词：微小RNA-21对人脐血间充质干细胞增殖和转化生长因子-β1表达的影响　出处：《安徽医科大学学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨微小RNA-21(miR-21)对人脐血间充质干细胞(MSC)增殖和转化生长因子(TGF)-β1表达的影响。方法体外分离并培养人脐血MSC,用LipofectamineTM2000转染miR-21模拟物(60μmol/L)、miR-21抑制剂(60μmol/L)和miR-21阴性对照(60μmol/L),空白对照组不做任何处理,MTT法分析对细胞增殖的影响;qRT-PCR和Western blot检测TGF-β1的表达。结果与空白对照组和阴性对照组相比,miR-21过表达明显抑制MSC增殖,沉默miR-21明显促进MSC增殖;此外,过表达miR-21靶向抑制细胞中TGF-β1的表达,而沉默miR-21可促进细胞中TGF-β1表达。结论 miR-21能抑制人脐血MSC的增殖,并下调其TGF-β1的表达。
[Abstract]:Objective to study the effect of RNA-21 (miR-21) on human umbilical cord blood mesenchymal stem cells (MSC) proliferation and transforming growth factor (TGF) - beta 1 expression. Methods in vitro isolated and cultured human umbilical cord blood MSC mimics transfected with LipofectamineTM2000 miR-21 (60 mol/L), miR-21 inhibitor (60 mol/L) and miR-21. The control (60 mol/L), blank control group without any treatment, analysis the effect on cell proliferation by MTT; expression of qRT-PCR and Western blot detection of TGF- beta 1. Results compared with the blank control group and negative control group, over expression of miR-21 significantly inhibited the proliferation of MSC, silencing of miR-21 significantly promote the proliferation of MSC; in addition, overexpression of miR-21 targeted inhibition of the expression of TGF- beta 1 cells, whereas knockdown of miR-21 can promote the cell TGF- beta 1 expression. Conclusion miR-21 can inhibit the proliferation of human umbilical cord blood MSC, and down-regulation of the expression of TGF- beta 1.

【作者单位】：安徽医科大学附属省立医院血液科;
【分类号】：R329.2
【正文快照】： 间充质干细胞(mesenchymal stem cell,MSC)具有多向分化的潜能,可以在体外诱导分化成骨细胞、神经细胞、心肌细胞等,是组织工程种子细胞的重要来源[1]。而相对于人骨髓MSC,人脐血MSC作为组织工程种子细胞的来源更为理想。微小RNAs(mi-croRNAs,miRs)是一类长为22~25个核苷酸序

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