体外定向诱导人羊膜间充质干细胞向骨、软骨及脂肪细胞的分化

发布时间：2018-01-07 02:24

本文关键词：体外定向诱导人羊膜间充质干细胞向骨、软骨及脂肪细胞的分化　出处：《中国组织工程研究》2017年01期 　论文类型：期刊论文

【摘要】：背景:人羊膜间充质干细胞属于成体干细胞,在体外不同条件下诱导分化为骨、软骨及其他结缔组织,较其他类型间充质干细胞,因其来源广泛,分离无创伤,较低的免疫原性、生长周期短等特点,是组织工程种子细胞的重要来源。目的:探讨人羊膜间充质干细胞是否具有间充质干细胞特性及诱导成骨、软骨及脂肪细胞诱导分化的能力。方法:取足月产胎盘羊膜组织,酶消化法分离人羊膜间充质干细胞,取第3代细胞分别加入成骨、成软骨及成脂诱导液的培养基进行诱导。结果与结论:(1)茜素红染色显示,成骨诱导后细胞存在多个矿化结节,细胞中碱性磷酸酶活性明显增加;(2)甲苯胺蓝染色显示,成软骨诱导后细胞基质中分泌大量的糖胺聚糖;(3)油红O染色显示,成脂诱导后,细胞浆内可见脂滴;(4)实时荧光PCR结果显示,成骨、成软骨及成脂诱导21 d后,相关基因表达较7 d时明显增高;(5)结果人羊膜间充质干细胞具有向成骨细胞,软骨细胞及脂肪细胞的潜能,可选作为组织工程学的种子细胞。
[Abstract]:Background: human amniotic mesenchymal stem cells (HMMSCs) are adult stem cells, which are differentiated into bone, cartilage and other connective tissues under different conditions in vitro. Compared with other types of mesenchymal stem cells, human amniotic mesenchymal stem cells are isolated without trauma because of their wide range of origin. Low immunogenicity and short growth cycle are important sources of seed cells for tissue engineering. Objective: to investigate whether human amniotic mesenchymal stem cells have the characteristics of mesenchymal stem cells and induce osteogenesis. Methods: human amniotic mesenchymal stem cells were isolated by enzyme digestion and the third passage cells were added to osteogenesis. Results and conclusion: alizarin red staining showed that there were many mineralized nodules and alkaline phosphatase activity in osteoblasts after osteogenesis induction. (2) toluidine blue staining showed that a large amount of glycosaminoglycan was secreted in the matrix after chondrogenic induction. Oil red O staining showed that lipid droplets could be seen in the cytoplasm of the cells induced by fat-forming. The results of real-time fluorescence PCR showed that the expression of related genes was significantly higher after 21 days of osteogenesis, cartilage formation and lipogenesis than at 7 days. Results the human amniotic mesenchymal stem cells had the potential of osteoblasts, chondrocytes and adipocytes, and could be used as seed cells for tissue engineering.
【作者单位】：遵义医学院附属医院骨科;
【基金】：贵州省科学技术基金资助项目(黔科合SY字[2010]3091)~~
【分类号】：R329.2
【正文快照】： 文章快速阅读:定向诱导人羊膜间充质干细胞向成骨、软骨及脂肪细胞分化的过程原代人羊膜间充第人羊膜酶消化传代培养3代人羊膜间质干细胞充质干细胞证实人羊膜间充质干细胞可以向成骨、软骨、脂肪细胞分化,成骨、软骨、且21 d的分化效果及相关基因表达较7 d时更优脂肪诱导文题

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