清热活血方对溃疡性结肠炎模型小鼠β-catenin、Cdx1、Cdx2表达影响的研究

发布时间：2018-01-07 21:01

本文关键词：清热活血方对溃疡性结肠炎模型小鼠β-catenin、Cdx1、Cdx2表达影响的研究　出处：《现代中西医结合杂志》2016年11期 　论文类型：期刊论文

【摘要】：目的观察清热活血方对溃疡性结肠炎模型小鼠结肠黏膜组织中β-catenin、Cdx1、Cdx2表达的影响,探讨该方防治溃疡性结肠炎的可能机制及其效应靶点。方法将100只雄性Balb/c小鼠随机分为正常组、模型组、清热活血方低剂量组、清热活血方中剂量组及清热活血方高剂量组,除正常组外,其余组采用自由饮用3%DSS 2周法制备溃疡性结肠炎模型。自第3周起,正常组、模型组给予等剂量生理盐水,各给药组分别给予清热活血方3.3g/kg、6.6 g/kg、13.2 g/kg灌胃,均连续1周。末次给药后处死全部小鼠,应用HE染色法检测结肠黏膜组织病理学变化;应用免疫组化及荧光定量PCR技术检测结肠黏膜组织中β-catenin、Cdx1、Cdx2表达情况;应用免疫荧光染色法检测结肠黏膜组织中β-catenin核内转位变化。结果光镜下可见模型组结肠黏膜上皮脱落,大量炎性细胞浸润及溃疡形成;而各给药组结肠黏膜充血、肿胀、上皮细胞脱落及成纤维母细胞表达增加,黏膜固有层可见少许炎性细胞浸润,未见明显溃疡。与正常组比较,模型组小鼠结肠黏膜中β-catenin、Cdx1、Cdx2表达明显增加(P均0.05);与模型组比较,各给药组β-catenin、Cdx1、Cdx2阳性表达明显减少(P均0.05)。模型组小鼠结肠黏膜中β-catenin存在膜表达缺失、核内异常转位现象,而各给药组小鼠结肠黏膜中β-catenin表达以细胞膜为主,核内表达减少,部分可见核内异常转位。结论清热活血方可能通过促进β-catenin降解,下调Cdx1、Cdx2表达,调控肠上皮细胞增殖,修复肠黏膜屏障而发挥缓解溃疡性结肠炎的效用。
[Abstract]:Objective to observe the effect of Qingre Huoxue recipe (QHR) on the expression of 尾 -cateninine cdx1cdx2 in colonic mucosa of mice with ulcerative colitis. Methods 100 male Balb/c mice were randomly divided into normal group, model group and low dose group of Qingre Huoxue recipe. The model of ulcerative colitis was established in the middle dose group of Qingre Huoxue prescription and the high dose group of Qingre Huoxue prescription except the normal group, the other groups were prepared by the method of free drinking 3DSS for 2 weeks. From the 3rd week, the normal group. The model group was given the same dose of normal saline, and each group was given the Qingre Huoxue prescription 3.3 g / kg ~ (6.6 g 路kg ~ (-1)) / kg ~ (13. 2) g 路kg ~ (-1) 路kg ~ (-1) respectively. All the mice were killed after the last administration, and the histopathological changes of colonic mucosa were detected by HE staining. Immunohistochemistry and fluorescence quantitative PCR were used to detect the expression of 尾 -cateninine CD _ (1) and CD _ (2) in colonic mucosa. The nuclear translocation of 尾 -catenin in colonic mucosa was detected by immunofluorescence staining. Results the colonic mucosal epithelium in the model group was shedded, a large number of inflammatory cells infiltrated and ulcer was formed under light microscope. However, the colonic mucosal congestion, swelling, epithelial cell shedding and fibroblast expression increased in each group, and a few inflammatory cells infiltrated in the lamina propria mucosa without obvious ulceration. In the model group, the expression of 尾 -cateninine CD _ (1) and CD _ (2) in colonic mucosa increased significantly (P < 0.05). Compared with the model group, 尾 -catenin cdx1 was used in each administration group. The expression of 尾 -catenin in colonic mucosa of mice in model group was absent and abnormal translocation in nucleus was observed. However, the expression of 尾 -catenin in colonic mucosa of mice treated with each drug was mainly cell membrane, and the expression of 尾 -catenin in nucleus was decreased. Conclusion Qingre Huoxue prescription may regulate the proliferation of intestinal epithelial cells by promoting the degradation of 尾 -catenin, down-regulating the expression of CD _ x1, CD _ X2 and regulating the proliferation of intestinal epithelial cells. Repair intestinal mucosal barrier to relieve ulcerative colitis.
【作者单位】：广东省江门市第二人民医院;广西中医药大学附属瑞康医院;
【基金】：广西自然科学基金资助项目(2013GXNSFAA011916) 江门市科技局资助项目(12A078)
【分类号】：R285.5;R-332
【正文快照】： 530011)生理情况下,结肠细胞凋亡主要发生在肠腔上皮细胞,而活动期溃疡性结肠炎患者,除肠腔上皮细胞外,病变处及邻近的非病变处隐窝上皮细胞的凋亡增加,从而使由上皮细胞构成的黏膜屏障破坏,导致了结肠黏膜的损伤、溃疡甚至癌变。目前越来越多的证据提示[1],结肠上皮细胞的凋

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