超声评价法优化三氯化铁诱导大鼠颈总动脉血栓模型的研究

发布时间：2018-01-09 12:25

  本文关键词：超声评价法优化三氯化铁诱导大鼠颈总动脉血栓模型的研究　出处：《中国神经精神疾病杂志》2017年02期 　论文类型：期刊论文

  更多相关文章： 动物模型 三氯化铁诱导血栓 闭塞血栓 超声评价

【摘要】：目的探讨采用连续超声评价法优化不同三氯化铁浓度梯度和作用时间诱导大鼠颈总动脉血栓模型,为溶栓及取栓研究提供合适的在体血栓模型。方法选取20只(Sprague Dawley)SD大鼠,每组5只,分别采用20%、30%、40%、50%四种三氯化铁浓度外敷大鼠单侧颈总动脉,使用14 L高频超声探头,分别在外敷10min、15 min、20 min三个时间点观察血管二维超声图像及彩色多普勒血流情况。选取血管闭塞最佳组大鼠,于干预120 min后观察血管自发再通率及静脉注射尿激酶后的血管再通率。实验结束后,取出干预血管甲醛固定,石蜡包埋,切片,进行HE染色病理分析。结果三氯化铁外敷大鼠颈总动脉20 min、50%浓度组血管闭塞率100%,40%、30%、20%浓度组外敷20 min血管闭塞率分别20%、0%、0%(P0.001)。外敷120 min后,50%浓度组组血管自发再通率0%、40%、30%、20%组血管自发再通率100%(P0.001),50%三氯化铁浓度造模组尾静脉注射尿激酶后血管部分再通率为40%。50%浓度组镜下病理显示血栓结构致密,尿激酶干预后管腔部分再通,血栓结构松散。结论采用50%三氯化铁浓度外敷大鼠颈总动脉20 min,可能是在体血栓模型形成的有效条件,该动物模型可能适合溶栓研究。
[Abstract]:Objective to study the continuous ultrasound evaluation method to optimize the rat common carotid artery thrombosis model with different ferric chloride concentration gradient and time of action. Methods Twenty Sprague Sprague Dawley)SD rats were selected in each group of 5 rats. 50% four kinds of ferric trichloride were applied to unilateral common carotid artery of rats with 14 L high frequency ultrasound probe for 10 minutes and 15 min respectively. Two dimensional ultrasound images and color Doppler flow were observed at three time points of 20 min. The best group of rats with vascular occlusion was selected. The spontaneous recanalization rate of blood vessel and the rate of vascular recanalization after intravenous injection of urokinase were observed after 120 min of intervention. After the experiment, the intervention vessel was fixed with formaldehyde, embedded in paraffin and sectioned. Results the vascular occlusion rate was 100% and 40% in the group of 20 min ~ 50% concentration of ferric chloride applied on the common carotid artery of rats. In the 20% group, the vascular occlusion rate for 20 min was 20 and the spontaneous recanalization rate was 0% in the 50% group after external application for 120 min. The spontaneous recanalization rate of blood vessels in 20% group was 100% (P 0.001). The vascular recanalization rate of the 50% ferric chloride concentration group was 40. 50% after urokinase was injected into the tail vein. The thrombus structure was dense and the lumen was partly recanalization after urokinase intervention in the group of 40. 50% concentration of ferric chloride. Conclusion the application of 50% ferric chloride to the common carotid artery for 20 min may be an effective condition for the formation of thrombus in vivo, and the animal model may be suitable for thrombolytic study.
【作者单位】： 北京大学第一医院;
【基金】：国家自然科学基金(编号:H0906)
【分类号】：R-332;R543.4
【正文快照】： 1990年,KURZ等[1],将三氯化铁外敷于大鼠颈总动脉诱导闭塞性血栓形成,同时发现血管闭塞时间依赖于三氯化铁溶液浓度,浓度越高,闭塞性血栓形成越快,浓度越低,闭塞血栓形成越慢。此方法形成的血栓为富含血小板、纤维蛋白和红细胞的混合型血栓,与临床自发性血栓组织形态学特征相，

本文编号：1401398