创伤性脑损伤合并骨折动物模型的建立与评估

发布时间：2018-01-09 13:02

  本文关键词：创伤性脑损伤合并骨折动物模型的建立与评估　出处：《医学研究生学报》2017年09期 　论文类型：期刊论文

  更多相关文章： 颅脑损伤 骨折 动物模型 加速

【摘要】：目的颅脑损伤与骨折愈合相关性机制研究缺乏确实可靠的动物实验模型。文章旨在建立创伤性脑损伤与骨折愈合相关性研究的动物模型,并进行稳定性评估。方法选择新西兰兔48只随机分为4组,每组12只:对照组(不做任何处理)、单纯颅脑外伤组(单纯颅脑损伤模型)、单纯骨折组(单纯制作右侧胫骨骨折模型)、脑外伤合并骨折组(制作颅脑损伤合并骨折模型)。观察各组新西兰兔神经行为学变化,并术后不同时间观察骨折愈合情况,行骨痂体积测量及病理学观察。结果单纯颅脑外伤组术后6 h、7 d、1个月神经功能量表(NSS)评分[(9.32±0.68)、(8.01±0.56)、(7.75±0.66)分]均显著低于对照组[(11.81±0.55)、(12.07±0.42)、(12.02±0.38)分],差异有统计学意义(P0.05)。与单纯骨折组术后1个月周围骨痂的体积比较,脑外伤合并骨折组明显增大[(1.19±0.12)cm~3vs(1.81±0.13)cm~3,P0.05]。脑外伤合并骨折组术后4周,骨折局部骨痂中骨小梁显著增多;术后8周骨小梁变得更加成熟。结论创伤性脑损伤合并骨折动物模型,稳定性及可重复性好,达到了脑损伤合并骨折相关研究所需的动物模型标准。
[Abstract]:Objective to establish an animal model of the relationship between traumatic brain injury and fracture healing in order to establish a reliable animal model for the study of the mechanism of craniocerebral injury and fracture healing. Methods 48 New Zealand rabbits were randomly divided into 4 groups, 12 in each group: control group (without any treatment), simple craniocerebral injury group (simple craniocerebral injury model). Simple fracture group (simple to make right tibial fracture model, brain injury combined with fracture group (craniocerebral injury combined with fracture model). To observe the changes of neurobehavioral changes in each group of New Zealand rabbits. The healing of fracture was observed at different time after operation, the volume of callus was measured and the pathological observation was made. Results the neurological function scale (NSS) was used in patients with craniocerebral trauma at 6 hours and 7 days after operation. [The score of 9.32 卤0.68 (8.01 卤0.56) was significantly lower than that of the control group (7.75 卤0.66). [(11.81 卤0.55) and 12.07 卤0.42 (12.02 卤0.38). The difference was statistically significant (P 0.05). Compared with the simple fracture group, the volume of peripheral callus was significantly larger in the group of traumatic brain injury with fracture than that in the group of simple fracture 1 month after operation. [The trabeculae in the local callus increased significantly 4 weeks after operation in the traumatic brain injury combined with fracture group (1.81 卤0.13 cm ~ (-3) P 0.05). Conclusion the animal model of traumatic brain injury combined with fracture is stable and reproducible, which meets the standard of animal model for the study of brain injury associated with fracture.
【作者单位】： 新疆医科大学第一附属医院骨科;
【基金】：新疆维吾尔自治区自然科学基金(2014211C045)
【分类号】：R-332;R651.15
【正文快照】： 0 引言 诊疗过程中经常发现大多数有骨折同时合并颅脑外伤的患者,其骨折局部新生骨痂的增长速度显著加快,并且骨痂超常生长,甚至肌肉组织中会呈现出异位骨化的现象,影响患者康复及功能锻炼[1-4]。1968年Roberts[5]首次报道表明脑外伤后身体局部会表现出异位骨化的现象,异位骨，

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