骨髓间充质干细胞移植对糖尿病模型大鼠肾脏的保护作用

发布时间：2018-01-09 15:11

本文关键词：骨髓间充质干细胞移植对糖尿病模型大鼠肾脏的保护作用　出处：《中国组织工程研究》2016年28期 　论文类型：期刊论文

【摘要】：背景:国内外关于干细胞移植对糖尿病慢性并发症的治疗作用已有较多理论研究,但其对糖尿病肾病的治疗作用尚无动物实验证实。目的:观察骨髓间充质干细胞移植对糖尿病模型大鼠肾脏的保护作用。方法:将大鼠用高糖高脂饮食配方喂养4周后注射链脲佐菌素,建立2型糖尿病模型大鼠,造模成功的第2天开始经尾静脉注射骨髓间充质干细胞单细胞悬液,并设对照组和糖尿病组大鼠作对照。细胞移植后第21天检查大鼠尾静脉血的血糖、三酰甘油和胰岛素水平,并进行肾脏和胰腺组织的病理学观察。结果与结论:(1)生化指标:糖尿病组大鼠血糖、血清胰岛素和三酰甘油水平均明显高于对照组(P0.05)。干细胞移植组大鼠血糖和血清胰岛素水平明显低于糖尿病组(P0.05);(2)肾脏和胰腺组织的病理学变化:干细胞移植组大鼠肾小球系膜面积与肾小球体积均明显小于糖尿病组(P0.05);(3)结果证实:骨髓间充质干细胞移植后可降低血糖及血清胰岛素水平,修复受损的胰腺及肾脏损伤。
[Abstract]:Background: there have been many theoretical studies on the therapeutic effect of stem cell transplantation on diabetic chronic complications at home and abroad. But its therapeutic effect on diabetic nephropathy has not been confirmed by animal experiments. Objective: to observe the protective effect of bone marrow mesenchymal stem cell transplantation on kidney of diabetic model rats. Rats were fed with high sugar and high fat diet for 4 weeks, then streptozotocin was injected. Type 2 diabetic model rats were established and the single cell suspension of bone marrow mesenchymal stem cells was injected through caudal vein on the second day of successful modeling. The levels of blood glucose triglyceride and insulin in caudal vein blood of rats were examined 21 days after transplantation. The pathological changes of kidney and pancreas were observed. Results and conclusion the biochemical index: blood glucose in diabetic rats. The levels of serum insulin and triglyceride were significantly higher than those of the control group (P 0.05). The levels of blood glucose and serum insulin in the stem cell transplantation group were significantly lower than those in the diabetic group. (2) pathological changes of kidney and pancreas: the glomerular Mesangial area and glomerular volume in the stem cell transplantation group were significantly smaller than those in the diabetic group (P 0.05); The results showed that bone marrow mesenchymal stem cells (BMSCs) could reduce blood glucose and serum insulin level and repair the damaged pancreas and kidney injury after bone marrow mesenchymal stem cell transplantation.
【作者单位】：武汉市中心医院内分泌科;
【分类号】：R587.2;R692.9;R-332
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