细粒棘球绦虫转酮醇酶生物信息学分析

发布时间：2018-01-09 18:29

本文关键词：细粒棘球绦虫转酮醇酶生物信息学分析　出处：《中国病原生物学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的利用生物信息学技术对EgTK基因编码蛋白的结构和功能进行预测和分析。方法利用ExPASy系统的ProtParam、ProtScale、SignalP、SOMPA程序与SubLoc v1.0、DNAStar、IEDB、SYPEITHI、Phyre2等生物信息学软件分析EgTK的理化性质、抗原表位、跨膜区、二、三级结构等。结果 EgTK基因由7个外显子,6个内含子构成,CDS长度为1 878bp,编码625个氨基酸,分子式为C_(3015)H_(4791)N_(829)O_(900)S_(22),分子质量为67.76ku;为跨膜蛋白,位于细胞质,二级结构主要以α螺旋、无规则卷曲为主。EgTK有16个潜在的B细胞线性表位,11个CTL细胞表位,13个Th细胞表位;EgTK三级结构与人类转酮醇酶相似性为97%;经多重比对,除去两端多余序列,共有473个氨基酸残基参与比对,发现195个变异位点、123简约性信息位点、72单态突变位点。结论生物信息学技术分析EgTK蛋白存在多个B、T细胞表位,具有良好的免疫原性,可为该蛋白的基因克隆、表达等提供理论依据。
[Abstract]:Objective to predict and analyze the structure and function of EgTK gene encoding protein by bioinformatics. Methods ProtParamia ProtScale of ExPASy system was used. The SignalPu SOMPA program and SubLoc v1.0 DNA starboard were used in the study of SYPEITHI. Phyre2 and other bioinformatics softwares were used to analyze the physicochemical properties, antigenic epitopes, transmembrane regions, secondary and tertiary structures of EgTK. Results the EgTK gene was composed of 7 exons and 6 introns. The length of the CDS is 1 878 BP and encodes 625 amino acids. Molecular weight is 67.76ku. It is a transmembrane protein located in the cytoplasm. The secondary structure is mainly 伪 helix. The irregular curl. EgTK has 16 potential B cell linear epitopes and 11 CTL cell epitopes. 13 Th cell epitopes; The similarity between the tertiary structure of EgTK and human transketol enzyme was 97%. After multiple alignment, 473 amino acid residues took part in the alignment, excluding the redundant sequences at both ends, and 195 variant sites were found to be parsimonious information sites. Conclusion the bioinformatics analysis of EgTK protein has many T cell epitopes and has good immunogenicity, which can provide theoretical basis for gene cloning and expression of EgTK protein.
【作者单位】：青海大学医学院病原生物学教研室;青海大学包虫病重点实验室;
【基金】：国家自然科学基金项目(No.81360255)
【分类号】：R383.33
【正文快照】： ***囊性包虫病(cystic echinococcosis,CE)是由细粒棘球绦虫(Echinococcus granulosus,Eg)的幼虫引起的一种在世界范围内广泛流行的寄生虫病,常见的感染途径是误食了感染细粒棘球绦虫的犬体内成虫排出的虫卵[1]。人感染后初期常多无症状。血清特异抗体常早于症状出现,但血清学

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