大鼠脾脏主要结构的发育——从出生至12月龄的形态学定量

发布时间：2018-01-10 07:02

本文关键词：大鼠脾脏主要结构的发育——从出生至12月龄的形态学定量　出处：《解剖学报》2017年03期 　论文类型：期刊论文

【摘要】：目的定量研究大鼠从出生至12月龄脾脏主要结构的发育情况。方法从同群正常雄性SD大鼠中,在不同发育阶段(1d和1、3、6、12个月)随机抽选动物,每个年龄组6~7只。取完整的脾脏,切取组织块制作甲基丙烯酸树脂包埋切片,经过碘酸-雪夫试剂和苏木素染色后用体视学方法定量研究脾内主要组织结构的总体积。结果从1d至1月龄,大鼠脾脏体积增长了6.7倍,从1月至3月又增长了2.0倍,从3至12月只增加了39%。脾脏在1日龄已形成动脉周围淋巴鞘,1月形成边缘区,3月形成脾小结,且脾血窦里充满血液。3~12月龄脾内白髓、红髓所占体积比例稳定,分别为23%~27%、67%~70%。结论大鼠脾脏在3月龄前生长快速,发育成熟,之后仍继续生长,但变化不明显。
[Abstract]:Objective to quantitatively study the development of the main structure of spleen from birth to 12 months of age in rats. The animals were randomly selected for 12 months. Seven animals were selected from each age group. The whole spleen was taken and tissue blocks were cut out to make methacrylic resin embedded sections. The total volume of main tissue structure in spleen was quantitatively studied by stereological method after iodine-Scheffer reagent and hematoxylin staining. Results the volume of spleen increased 6.7 times from 1 day to 1 month of age. From January to March, there was a 2.0-fold increase. From 3 to December, the spleen increased only 39 times. The spleen formed periarterial lymphatic sheath at 1 day of age, marginal area in December and splenic nodules in December. The splenic sinuses were filled with white pulp in the spleen at 12 months of age, and the proportion of the red pulp in the spleen was stable. The volume ratio of the red pulp was 232777.70. conclusion the spleen of the rats grows rapidly before 3 months of age. After maturing, it continued to grow, but the change was not obvious.
【作者单位】：川北医学院动物发育研究室;川北医学院实验动物中心;川北医学院形态定量研究室;
【基金】：川北医学院科研发展计划项目(CBY13-A-ZP09、CBY13-A-ZP05)
【分类号】：R33
【正文快照】： 脾脏是重要的免疫器官,早期的脾脏还有造血功能,至3月龄时脾内各种结构已具有典型组织学形态[1~3]。文献很少报道大鼠脾脏的生长发育情况[3],脾内组织结构的定量研究更是缺乏。我们拟研究正常大鼠脾脏1年的生长发育情况,用体视学方法定量研究脾内主要组织结构总体积的变化,为

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