侧脑室注射apelin-13抑制小鼠远端结肠运动

发布时间：2018-01-10 16:10

本文关键词：侧脑室注射apelin-13抑制小鼠远端结肠运动　出处：《兰州大学》2011年硕士论文　论文类型：学位论文

【摘要】：Apelin及其受体APJ广泛分布于中枢神经系统和外周组织。在周围组织包括脾、胸腺、心脏、乳腺、胃、小肠和结肠等多个器官内都能检测到APJ受体和apelin的mRNA。在中枢神经系统,APJ以及apelin的mRNA主要分布于下丘脑、丘脑、大脑皮层和垂体等与内脏运动紧密相关的脑区。并且apelin对多种机体的生理和病理活动具有调节作用,包括心血管、免疫、神经内分泌、体液平衡和摄食等。许多研究结果表明摄食与胃肠运动功能关系密切,提示apelin可能与胃肠运动的调节有关,然而目前尚未见相关报道。本研究旨在探讨侧脑室注射apelin-13对小鼠结肠运动的影响以及apelin-13对离体结肠收缩的影响。侧脑室注射apelin-13 (0.3,0.5,1和3μg/只)能够剂量依赖的抑制结肠排珠和排便,说明apelin-13在中枢水平上参与结肠运动的调控。为了探讨apelin-13抑制结肠运动的机制,我们选用APJ受体特异性拮抗剂apelin-13 (F13A)进行研究,发现其能有效逆转apelin-13的上述作用,说明APJ受体参与介导apelin-13对结肠运动的抑制作用。为了进一步探讨apelin-13的作用机制,我们选取非选择性阿片受体拮抗剂纳洛酮,结果证明它也能够完全阻断apelin-13对结肠运动的抑制作用,说明阿片受体也参与了这一调制过程。然而离体实验结果表明(10-8-10-6M) apelin-13并不能引起远端结肠肌条的收缩变化。
[Abstract]:Apelin and its receptor APJ are widely distributed in the central nervous system and peripheral tissues, including the spleen, thymus, heart, breast, stomach. APJ receptors and apelin mRNAs can be detected in many organs such as small intestine and colon. APJ and mRNA of apelin are mainly distributed in hypothalamus and thalamus in central nervous system. The cerebral cortex and pituitary are closely related to the visceral movement, and apelin can regulate the physiological and pathological activities of many organisms, including cardiovascular, immune, neuroendocrine. Many studies have shown that intake is closely related to gastrointestinal motility, suggesting that apelin may be involved in the regulation of gastrointestinal motility, but no related reports have been reported. The purpose of this study was to investigate the effects of intracerebroventricular injection of apelin-13 on colonic motility in mice and the effect of apelin-13 on colonic contraction in vitro. 0.3. 0. 5 渭 g / g and 3 渭 g / g) could inhibit colon droplet excretion and defecation in a dose-dependent manner. It is suggested that apelin-13 is involved in the regulation of colonic motility at the central level. In order to explore the mechanism of apelin-13 inhibiting colon motility. We selected apelin-13 F13A, a specific antagonist of APJ receptor, and found that it can effectively reverse the above effects of apelin-13. It is suggested that APJ receptor is involved in mediating the inhibitory effect of apelin-13 on colon motility. In order to further explore the mechanism of apelin-13. We selected naloxone, a non-selective opioid receptor antagonist, which proved that naloxone could also completely block the inhibition of colon motility by apelin-13. These results suggest that opioid receptors are also involved in this modulation process. However, in vitro, 10-8-10 ~ (-6) M) apelin-13 can not induce the contraction of distal colonic muscle strips.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

【参考文献】