白术七物颗粒剂对气阴两虚型慢传输型便秘模型大鼠结肠组织c-kit的影响及其作用机制

发布时间：2018-01-10 23:11

本文关键词：白术七物颗粒剂对气阴两虚型慢传输型便秘模型大鼠结肠组织c-kit的影响及其作用机制　出处：《中国全科医学》2017年21期 　论文类型：期刊论文

【摘要】：目的研究白术七物颗粒剂对气阴两虚型慢传输型便秘(STC)模型大鼠结肠组织c-kit的影响,探讨其可能作用机制。方法 2015年9—11月,将70只SPF级SD大鼠随机分成空白组20只、模型组50只,雌雄各半。模型组大鼠皮下注射盐酸吗啡注射液2.5 mg/kg,空白组注射等量0.9%氯化钠溶液,1次/d,连续注射45 d。模型组大鼠皮下注射盐酸吗啡注射液15 d后,灌服(青皮、枳壳、附子,剂量13 g/kg),连续30 d,建立气阴两虚型STC大鼠模型。模型建立成功后,将模型组剩下的40只大鼠随机分为模型对照组,白术七物颗粒高、低剂量组和莫沙必利组,每组10只,雌雄各半。除空白组、模型对照组外,白术七物颗粒高、低剂量组和莫沙必利组给予相应的药物灌胃。应用RT-PCR、免疫组化法检测白术七物颗粒剂对气阴两虚型STC模型大鼠结肠组织c-kit影响。结果模型组样本大鼠肠道推进率低于空白组样本(P0.05)。白术七物颗粒低剂量组、白术七物颗粒高剂量组、莫沙必利组大鼠小肠推进率高于模型对照组(P0.05)。白术七物颗粒高剂量组、莫沙必利组c-kit mRNA表达水平高于模型对照组(P0.05)。白术七物颗粒低剂量组c-kit mRNA表达水平低于白术七物颗粒高剂量组(P0.05)。白术七物颗粒高剂量组、莫沙必利组c-kit表达水平高于模型对照组(P0.05)。结论白术七物颗粒剂治疗气阴两虚型STC模型大鼠的作用机制是通过增加结肠组织中c-kit表达水平来实现的。
[Abstract]:Objective to study the effect of Atractylodes macrocephala granule on c-kit in colon tissue of rats with slow transit constipation of deficiency of Qi and Yin and to explore its possible mechanism. Methods from September to November, 2015. Seventy SPF SD rats were randomly divided into blank group (n = 20), model group (n = 50), male and female (n = 50). Rats in model group were subcutaneously injected with morphine hydrochloride injection for 2.5 mg/kg. Rats in the model group were injected with morphine hydrochloride injection for 15 days after subcutaneous injection of morphine hydrochloride for 15 days, and were administered with green peel, Fructus Aurantii and Aconitum aconite (Fructus aurantii, Fructus aurantii, Aconitum aconite). The model of STC rats with deficiency of both qi and yin was established at a dose of 13 g / kg for 30 days. After the establishment of the model, the remaining 40 rats in the model group were randomly divided into the model control group. Atractylodes Atractylodes Qiwu granules were high, low dose group and mosapride group, 10 rats in each group, half male and female. Except blank group and model control group, Atractylodes macrocephala seven objects granules were high. The low dose group and mosapride group were given intragastric administration of corresponding drugs. RT-PCR was used. Immunohistochemical method was used to detect the effect of Atractylodes macrocephala on c-kit in colon tissue of STC model rats with deficiency of qi and yin. Results the intestinal propulsion rate in model group was lower than that in blank group (P 0.05). The low dose group of Atractylodes macrocephala Qiwu granules. The propelling rate of small intestine in the high dose group and mosapride group was higher than that in the model control group (P 0.05). The expression of c-kit mRNA in mosapride group was higher than that in model control group (P0.05). The expression of c-kit mRNA in low dose group was lower than that in high dose group (P 0.05). The expression of c-kit in mosapride group was higher than that in model control group (P0.05). Conclusion the mechanism of Atractylodes macrocephala granule in treating STC rats with deficiency of qi and yin is achieved by increasing the expression of c-kit in colon tissue.
【作者单位】：湖南中医药大学第二附属医院肛肠科;湖南省斯莱克景达实验动物有限公司;
【基金】：国家自然科学基金资助项目(81302977) 湖南省自然科学基金资助项目(13JJ3101)
【分类号】：R285.5;R-332
【正文快照】： 1.410005湖南省长沙市,湖南中医药大学第二附属医院肛肠科2.410100湖南省长沙市,湖南省斯莱克景达实验动物有限公司作用机制[J].中国全科医学,2017,20(21):2640-2643,2648.[www.chinagp.net]LIN R J,LU H Y,HE Y H,et al.Effect and mechanism of Atractylodes qiwu Granules

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