提高免疫组织化学反应的抗原修复技术

发布时间：2018-01-10 23:20

本文关键词：提高免疫组织化学反应的抗原修复技术　出处：《重庆医学》2016年04期 　论文类型：期刊论文

【摘要】：目的探讨不同抗原修复方法对免疫组织化学反应结果的影响,以提高免疫组织化学染色阳性率。方法分别采用3种不同抗原修复方法作预处理后,进行Envision免疫组织化学二步法检测10种常用抗体的免疫组织化学染色效果。结果石蜡组织切片,免疫组织化学反应的抗原修复方法预处理,采用高温高压抗原修复法,与微波抗原修复法及煮沸抗原修复法染色强度有明显差异,高温高压抗原修复法明显优于微波法及煮沸法。结论预处理采用高温高压抗原修复法,既可提高免疫组织化学反应阳性表达率,又能提高制片质量,可以一次性用于大量组织切片,是一种理想的免疫组织化学反应的抗原修复预处理方法。
[Abstract]:Objective to investigate the effect of different antigen repair methods on the results of immunohistochemistry in order to increase the positive rate of immunohistochemical staining. Envision immunohistochemical two-step method was used to detect the immunohistochemical staining effect of 10 kinds of antibodies. Results paraffin sections and antigen repair methods of immunohistochemistry were pretreated. The staining intensity of high temperature and high pressure antigen repair method was significantly different from microwave antigen repair method and boiled antigen repair method. High temperature and high pressure antigen repair method is obviously superior to microwave method and boiling method. Conclusion Pretreatment with high temperature and high pressure antigen repair method can not only improve the positive expression rate of immunohistochemistry, but also improve the quality of film preparation. It can be used in a large number of tissue sections at one time. It is an ideal antigen repair pretreatment method for immunohistochemistry.
【作者单位】：重庆市肿瘤研究所病理科;重庆市肿瘤研究所检验科;
【分类号】：R361.2
【正文快照】： 免疫组织化学技术已成为病理科实验室的常规工作及科研工作中不可缺少的重要手段,广泛应用于临床病理诊断[1],帮助评价肿瘤的增生程度和分期,对患者预后、治疗等方面起到了指导作用。但在日常工作中病理标本都用10%中性福尔马林液固定,经过10%中性福尔马林液固定的石蜡组织,会

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