组蛋白乙酰化修饰在2种不同方法建立小鼠心肌肥厚模型中的作用

发布时间：2018-01-12 05:04

本文关键词：组蛋白乙酰化修饰在2种不同方法建立小鼠心肌肥厚模型中的作用　出处：《临床心血管病杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:探讨组蛋白乙酰化修饰失衡在2种不同方法建立小鼠心肌肥厚模型中的作用。方法:选取昆明小鼠为研究对象,按照随机数字表法随机分为5组:正常组、0.9%氯化钠溶液组、苯肾上腺素组、手术组和假手术组。苯肾上腺素组给予苯肾上腺素皮下注射,手术组给予部分结扎腹主动脉建立小鼠心肌肥厚模型,实时荧光定量聚合酶链反应(real-time polymerase chain reaction,RT-PCR)检测心肌肥厚相关标志物心房利钠肽(atrial natriuretic peptide,ANP)及β-肌球蛋白重链(β-myosin heavy chain,β-MHC)mRNA表达水平,免疫印迹(western blot,WB)检测小鼠心肌组织中组蛋白H3赖氨酸残基9位乙酰化(H3K9ac)的表达,比色法检测心肌组织中组蛋白乙酰化酶(histone acetylases,HATs)、组蛋白去乙酰化酶(histone deacetylases,HDACs)活性,超声心动图观察小鼠心肌肥厚情况。结果:RT-PCR结果表明苯肾上腺素组、手术组小鼠心肌组织中ANP和β-MHC mRNA表达水平分别显著高于0.9%氯化钠溶液组、假手术组(P0.05);超声心动图结果显示苯肾上腺素组、手术组小鼠室间隔厚度、左室前壁厚度分别显著高于0.9%氯化钠溶液组、假手术组(P0.05),而左室舒张末期直径则分别显著低于0.9%氯化钠溶液组、假手术组(P0.05)。Western blot及比色法结果显示:苯肾上腺素组、手术组小鼠心肌组织中组蛋白H3K9ac的乙酰化水平及HATs活性分别显著高于0.9%氯化钠溶液组、假手术组(P0.05),而HDACs活性则分别显著低于0.9%氯化钠溶液组、假手术组(P0.05)。结论:组蛋白乙酰化修饰失衡均参与了2种不同方式所致的小鼠心肌肥厚。
[Abstract]:Objective: to investigate the role of histone acetylation modification imbalance in the establishment of myocardial hypertrophy model in mice by two different methods. Methods: Kunming mice were selected as study objects. According to the method of random number table, the rats were randomly divided into five groups: the normal group with 0.9% sodium chloride solution, the phenylephrine group, the operation group and the sham operation group, and the phenylephrine group was subcutaneously injected with phenylephrine. The model of myocardial hypertrophy was established by partial ligation of abdominal aorta in the operation group. Real-time polymerase chain reaction. The atrial natriuretic peptide (atrial natriuretic peptide) natriuretic peptide was detected by RT-PCR. The expression levels of 尾 -myosin heavy chain (尾 -MHCN) and 尾 -myosin heavy chain (尾 -MHCN) were detected by Western blot western blot. The expression of histone H3 lysine residues in the myocardium of mice was detected by WB). Histone acetylasesse (HATs) was detected by colorimetric method. Histone deacetylasesus HDACsactivity. Results the myocardial hypertrophy of mice was observed by echocardiography. Results the results showed that the phenylephrine group was the group of phenylephrine. The expression levels of ANP and 尾 -MHC mRNA in myocardium of the operation group were significantly higher than those of the 0.9% sodium chloride solution group and the sham operation group respectively. The results of echocardiography showed that the ventricular septal thickness and left ventricular anterior wall thickness in the phenylephrine group and the operation group were significantly higher than those in the 0.9% sodium chloride solution group and the sham operation group respectively (P 0.05). However, left ventricular end-diastolic diameter was significantly lower than that of 0.9% sodium chloride solution group. The results of P0.05 blot and colorimetry showed that: phenylephrine group. The acetylation level and HATs activity of histone H3K9ac in myocardium of the operation group were significantly higher than those of the 0.9% sodium chloride solution group and the sham operation group (P 0.05). The activity of HDACs was significantly lower than that of 0.9% sodium chloride solution group. Conclusion: the imbalance of histone acetylation modification is involved in myocardial hypertrophy induced by two different ways in mice.
【作者单位】：遵义医学院基础医学院生理教研室;遵义医学院第一临床学院临床系;遵义医学院附属医院儿内一科;
【基金】：国家自然科学基金项目(No:81560040) 遵义医学院博士启动基金项目[院字(2015)4号] 遵义医学院医学与科技学院大学生创新训练项目[遵医科院(2015)3108]
【分类号】：R-332;R542.2
【正文快照】： 心肌肥厚是多种心脏疾患的一个重要病理过氯化钠溶液组给予相应剂量的0.9%氯化钠溶液皮程,最终将发展为严重心力衰竭。心肌肥厚动物模下注射;正常组未予任何处理。建模成功后收集小型则是替代人体研究较为理想的研究模式。心肌鼠心脏放入-80℃冰箱保存备用。肥厚动物模型的构

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