肝细胞生长因子对大鼠背部超长任意皮瓣存活率影响的实验研究

发布时间：2018-01-12 06:08

本文关键词：肝细胞生长因子对大鼠背部超长任意皮瓣存活率影响的实验研究　出处：《中国美容医学》2011年03期 　论文类型：期刊论文

【摘要】：目的:探讨肝细胞生长因子对大鼠背部超长任意皮瓣血管生成的作用及皮瓣存活率的影响。方法:建立大鼠背部超长任意皮瓣8.0cm×2.0cm动物模型,直接皮下注射50ng/ml肝细胞生长因子溶液,术后7天观察皮瓣颜色、质地、毛细血管回流、坏死范围、切割皮瓣出血情况;计算皮瓣的存活面积;免疫组化染色后在显微镜下计算毛细血管平均密度。通过自身前后对照、与生理盐水组对照,比较两者的差异。结果:肝细胞生长因子组皮瓣的存活率81.45%±2.74%,与对照组的42.82%±7.03%比较,差别有统计学意义(P0.05);实验前实验组和对照组毛细血管数分别为12.70±1.35、12.31±1.22,两者差别无统计学意义(P0.05);实验后实验组和对照组毛细血管数分别为39.67±4.83、21.50±1.87,两者差别有统计学意义(P0.05);实验组及对照组实验后毛细血管密度高于实验前,差别有统计学意义(P0.05)。结论:肝细胞生长因子能促进皮瓣血管内皮细胞的生成,提高皮瓣的存活率。
[Abstract]:Objective: to investigate the effect of hepatocyte growth factor (HGF) on the angiogenesis and the survival rate of ultrashort dorsal flaps in rats. An animal model of 8. 0 cm 脳 2. 0 cm arbitrary skin flap was established. 50 ng / ml hepatocyte growth factor (HGF) solution was injected subcutaneously. The color, texture, capillary reflux, necrotic range and bleeding of the cut flap were observed 7 days after operation. The survival area of the flap was calculated. The mean capillary density was calculated under microscope after immunohistochemical staining. Results: the survival rate of the flap in the hepatocyte growth factor group was 81.45% 卤2.74 and 42.82% 卤7.03% in the control group. The difference was statistically significant (P 0.05). Before the experiment, the number of capillaries in the experimental group and the control group were 12.70 卤1.35 and 12.31 卤1.22, respectively. There was no significant difference between the two groups (P 0.05). After the experiment, the number of capillaries in the experimental group and the control group were 39.67 卤4.83 卤21.50 卤1.87, respectively. The difference between the two groups was statistically significant (P 0.05). The capillary density of experimental group and control group was higher than that of before experiment, the difference was statistically significant (P 0.05). Conclusion: hepatocyte growth factor can promote the formation of vascular endothelial cells of skin flap. To improve the survival rate of the flap.
【作者单位】：广西医科大学第一附属医院烧伤整形科;
【分类号】：R622
【正文快照】： 基础研究皮瓣修复是创伤(烧伤)、整形外科常用的治疗手段,传统的任意皮瓣的长宽之比为(2.0～1.5)∶1,超过此比例皮瓣远端常常会发生坏死导致手术失败而需要再次手术,给病人及家属带来痛苦和经济负担。目前临床上常用丹参注射液、低分子右旋糖酐及前列腺素E1、罂粟碱等通过疏

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