3种培养基中芽囊原虫生长状况观察

发布时间：2018-01-12 17:03

本文关键词：3种培养基中芽囊原虫生长状况观察　出处：《中国血吸虫病防治杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的观察芽囊原虫在不同培养基中的生长状况,筛选合适的芽囊原虫培养方法。方法将10份芽囊原虫阳性粪便分别接种至Jone’s液、vitro液、1640 3种培养基中培养,选取生长情况良好、虫密度高的一份粪便传代培养后等量接种至3种不同的培养基中,每24 h计数虫体数量一次,连续观察10 d,并观察芽囊原虫的形态学变化和生长情况。结果10份粪便分别接种至3种培养基中培养,48 h后发现1640和Jone’s液中的虫密度高于vitro液。同一份粪便接种至3种不同培养基中连续观察10 d,结果表明3种培养基中虫体生长呈规律变化,均在接种后3、6、9 d出现生长高峰;Jone’s液中芽囊原虫密度最高。vitro液中观察到的虫体形态最清晰,且富有活力;Jone’s液中能观察到虫体多种繁殖状态。结论Jone’s液适于芽囊原虫的生长繁殖,可作为首选培养基;vitro液可作为观察芽囊原虫生长情况的首选培养基。
[Abstract]:Objective to observe the growth of Bud cyst protozoa in different media and to screen suitable culture methods. Methods 10 feces were inoculated into Jone's solution. In 1640 3 kinds of culture medium, one piece of feces with good growth condition and high insect density was selected and inoculated into 3 different culture media in equal quantity, and the number of insect bodies was counted once every 24 hours. The morphological changes and growth of Bud cyst protozoa were observed for 10 days. Results 10 feces were inoculated into three kinds of culture medium. 48 h later, it was found that the density of worms in 1640 and Jone's solution was higher than that in vitro solution. The same feces were inoculated into three different media for 10 days. The results showed that there was a regular change in the growth of the insect in the three kinds of culture medium, and the growth peak appeared on the 9th day after inoculation. The highest density of budding protozoa was observed in Jone's solution. Conclusion Jone's solution is suitable for the growth and reproduction of Bud cyst protozoa and can be used as the preferred medium. Vitro solution can be used as the preferred medium for observing the growth of Bud cyst protozoa.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所世界卫生组织疟疾、血吸虫病和丝虫病合作中心卫生部寄生虫病原与媒介生物学重点实验室;
【基金】：国家自然科学基金(81473022) 上海市卫计委科研课题(140826120622123) 国家科技重大专项(2012ZX1004-220)
【分类号】：R382
【正文快照】： 芽囊原虫(Blastocystis sp.)是一种单细胞厌氧原虫,寄生在人和哺乳动物肠道[1],该虫呈世界性分布,在热带地区和发展中国家感染率较高[2]。关于该原虫的致病性目前仍存在争议,一般认为其致病力较弱,是引起人类腹泻的致病因素[3],多数感染者无临床症状,但免疫力低下者和HIV患者

【相似文献】