肥胖小鼠良性前列腺增生模型的建立

发布时间：2018-01-13 10:54

  本文关键词：肥胖小鼠良性前列腺增生模型的建立　出处：《中国老年学杂志》2017年19期 　论文类型：期刊论文

  更多相关文章： 肥胖小鼠 良性前列腺增生 前列腺体积 前列腺指数

【摘要】：目的构建肥胖小鼠良性前列腺增生的动物模型。方法 SPF级C57BL/6J小鼠28只,取8只小鼠作为对照组(C组)喂养普通饲料,剩余的20只小鼠为高脂诱导肥胖组(Dio组)喂食高脂饲料,构建肥胖小鼠良性前列腺增生的模型。酶联免疫法检测血糖血脂、血清雌二醇(E2)和睾酮(T);水取代法检测前列腺体积;HE染色检测前列腺组织病理变化;IPP图像分析软件统计前列腺腺腔面积和上皮高度。结果实验第9周,高脂喂养后有12只小鼠达到肥胖标准(为Ob组),肥胖率为60%。Ob组小鼠体重、内脏脂肪重量明显大于C组(P0.05),前列腺重量、前列腺指数、体积、面积和上皮高度明显大于C组(P0.05);Ob组E2水平高于C组(P0.05),T水平低于C组(P0.05),而雌雄激素(E2/T)比值Ob组大于C组(P0.05);前列腺组织病理切片显示Ob组小鼠前列腺发生增生的病理变化。结论肥胖小鼠发生了前列腺增生。
[Abstract]:Objective to construct the animal model of benign prostatic hyperplasia in obese mice. Methods SPF 28 C57BL/6J mice, 8 mice as control group (group C) were fed with common feed, the remaining 20 mice for high fat induced obesity group (Dio group) were fed a high-fat diet, construct obese mice benign prostatic hyperplasia model detection. Blood glucose and lipid ELISA, serum estradiol (E2) and testosterone (T); water substitution method detection of prostate volume; HE staining was used to detect the pathological changes of prostate tissue; IPP image analysis software statistical prostate cavity area and epithelial height. The results of experiment ninth weeks after high-fat feeding 12 mice obese (for the Ob group), the obesity rate was 60%.Ob mice body weight, visceral fat weight was significantly higher than that of C group (P0.05), prostate weight, prostate index, volume, area and epithelial height was significantly higher than that of C group (P0.05); the level of E2 Ob was higher than that of C group (P0.05), T Compared with group C (P0.05), the ratio of sex hormones (E2/T) in group Ob was larger than that in group C (P0.05), and pathological changes in prostate tissue showed that there was a pathological change in hyperplasia of prostate in Ob group.

【作者单位】： 上海体育学院上海市人类运动能力开发与保障重点实验室运动健身科技省部重点实验室;
【基金】：国家自然科学基金资助项目(81472148) 上海市体育局科技综合计划项目(Z022)
【分类号】：R-332;R697.3
【正文快照】： 良性前列腺增生(BPH)是老年男性最常见的疾病之一,年龄、生活方式等是BPH的重要原因,60~69岁的男性BPH发病率为70%,70岁以上的男性BPH的发病率为80%[1]。因此,探索BPH的发病机制,对提高老年男性的生活质量具有重大意义。国内外多项研究发现肥胖与BPH密切相关,体重、身高、腰围，

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