内皮克隆形成细胞条件培养基对人真皮成纤维细胞生物学作用的影响

发布时间：2018-01-13 10:55

本文关键词：内皮克隆形成细胞条件培养基对人真皮成纤维细胞生物学作用的影响　出处：《实用医学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨内皮克隆形成细胞条件培养基(ECFCs-CM)对人真皮成纤维细胞(HDFs)生物学作用的影响。方法:从人脐带血中分离内皮克隆形成细胞(ECFCs)并鉴定;采用抗体芯片检测ECFCs-CM中细胞因子表达谱;将ECFCs-CM作用于HDFs,EBM-2作为对照,采用CCK-8法、细胞划痕实验及流式细胞术分别检测细胞的增殖、迁移及凋亡情况。结果:原代培养细胞符合ECFCs的特征;ECFCs-CM相对高表达PDGF-BB、EGF等细胞因子;实验组HDFs增殖能力和迁移能力均高于对照组,且在血清饥饿环境下实验组HDFs凋亡率低于对照组。结论:ECFCs-CM可促进HDFs增殖和迁移,并可抑制其在血清饥饿环境下的凋亡。
[Abstract]:Objective: to investigate the effect of ECFCs-CM) on human dermal fibroblasts (HDFs). Methods: ECFCswere isolated from human umbilical cord blood and identified. The cytokine expression profile in ECFCs-CM was detected by antibody chip. The proliferation of HDFsN EBM-2 cells was detected by CCK-8 method, cell scratch assay and flow cytometry. Results: the primary cultured cells accorded with the characteristics of ECFCs. The expression of PDGF-BBEGF and other cytokines was relatively high in ECFCs-CM. The proliferative ability and migration ability of HDFs in the experimental group were higher than those in the control group. The apoptosis rate of HDFs in the experimental group was lower than that in the control group under the condition of serum starvation. Conclusion: ECFCs-CM can promote the proliferation and migration of HDFs and inhibit its apoptosis under the condition of serum starvation.
【作者单位】：南方医科大学广州临床医学院;广州军区广州总医院整形外科;广东省中山市第二人民医院整形美容科;
【基金】：国家自然科学基金面上项目(编号:81571910) 广东省科技计划项目(编号:2014A02212256);广州市科技计划项目(编号:201607010394)
【分类号】：R329.2
【正文快照】： 国外研究发现内皮克隆形成细胞(endothelial及FITC-UEA-I结合能力;Matrigel基质胶成管试colony-forming cells,ECFCs)移植可促进糖尿病小验;流式细胞仪检测细胞表型,包括CD34、CD133、鼠创面的愈合[1],提示ECFCs移植在慢性创面的KDR、CD144、CD45。(2)HDFs原代培养:此部分参治

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