大鼠胃旁路手术模型建立及影响因素

发布时间：2018-01-14 01:10

本文关键词：大鼠胃旁路手术模型建立及影响因素　出处：《局解手术学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的建立SD大鼠胃旁路手术模型,分析SD大鼠胃旁路手术的影响因素及其预防措施,为进一步建立糖尿病大鼠(GK大鼠)胃旁路手术模型提供经验和技术积累。方法对30只SD大鼠实施胃旁路手术,分析影响建模成败的各种因素。结果大鼠建模成功17只,死亡13只,死亡率43.3%。3只大鼠在术中死亡,其中麻醉因素致死2只,心衰致死1只;1只因吻合口出血在术后2 h死亡;8只在术后第3天死亡,其中感染致死2只,小肠坏死致死2只,吻合口狭窄或梗阻致死4只;1只大鼠在术后第5天因误食固体食物致吻合口瘘死亡。本组大鼠无切口裂开等其他并发症。结论 SD大鼠胃旁路手术模型作为GK大鼠胃旁路手术模型的预实验是经济可行的。麻醉及围手术期管理、手术技巧、感染防治是建模成败的主要影响因素,加强相关因素的控制可以提高建模成功率。
[Abstract]:Objective to establish a model of gastric bypass surgery in SD rats, and to analyze the influencing factors and preventive measures of gastric bypass surgery in SD rats. Methods 30 SD rats were operated by gastric bypass in order to provide experience and technique accumulation for further establishing the model of gastric bypass surgery in diabetic rats. Results 17 rats were successfully established, 13 rats died, and 43.3 rats died during operation, 2 rats died due to anesthesia and 1 died from heart failure. 1 died of anastomotic bleeding only 2 hours after operation; 8 rats died on the 3rd day after operation, 2 died of infection, 2 died of small intestinal necrosis and 4 died of anastomotic stenosis or obstruction. On the 5th day after operation, one rat died of anastomotic fistula caused by accidental feeding of solid food. There were no other complications such as incision rupture in this group. SD rat gastric bypass operation model as a GK rat gastric bypass surgery model pre-experiment is economic and feasible, anesthesia and perioperative management. Surgical techniques and infection prevention are the main factors influencing the success or failure of modeling. Strengthening the control of related factors can improve the success rate of modeling.
【作者单位】：南方医科大学;广州军区广州总医院甲状腺糖尿病外科中心;
【基金】：广东省科技计划项目(2011B050400041) 广东省自然科学基金资助项目(S2011010000472)
【分类号】：R656.61;R-332
【正文快照】： 胃旁路手术作为治疗肥胖2型糖尿病(type 2 dia-betes mellitus,T2DM)的有效方法,2009年被美国糖尿病协会(American Diabetes Association,ADA)写入糖尿病治疗指南[1],目前在国外已广泛开展。中国刘金刚等[2]于2014年11月在《中国实用外科杂志》首次发布了“中国肥胖和2型糖尿

【相似文献】