自发性糖尿病长爪沙鼠Rxra在6种组织中的表达水平分析

发布时间：2018-01-14 03:20

本文关键词：自发性糖尿病长爪沙鼠Rxra在6种组织中的表达水平分析　出处：《中国比较医学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的分析糖尿病长爪沙鼠Rxra基因在6种组织中的表达水平,以期探索长爪沙鼠糖尿病发生的分子机制。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,分别采集动物的骨骼肌、肝脏、脂肪、肾脏、心脏和脑组织,用Real-time PCR和Western blotting检测Rxra基因在各组织中mRNA和蛋白表达水平。结果 Real-time PCR的结果表明,Rxra基因的mRNA水平在糖尿病组动物骨骼肌和脂肪中显著降低,在肝脏中无差异,而在肾脏、心脏和脑组织中则有高表达趋势。Western blotting结果显示,糖尿病组RXRA的蛋白水平在骨骼肌组织中显著降低,在脂肪组织几乎检测不到,在其他各组织表达情况有所不同,但没有统计学差异。结论 Rxra在糖尿病长爪沙鼠骨骼肌和脂肪组织中低表达,在其他4种组织中表达水平无差异,说明Rxra对长爪沙鼠糖尿病的影响主要发生在骨骼肌和脂肪组织中。
[Abstract]:Objective to analyze the expression level of Rxra gene in 6 tissues of diabetic gerbils in order to explore the molecular mechanism of diabetes. Methods six diabetic gerbils and six normal gerbils were selected. The skeletal muscle, liver, fat, kidney, heart and brain tissues of the animals were collected. Real-time PCR and Western blotting were used to detect the mRNA and protein expression of Rxra gene in all tissues. Results Real-time. PCR results show that. The mRNA level of Rxra gene was significantly decreased in skeletal muscle and fat in diabetic group, but not in liver, but in kidney. The results of Western blotting showed that the protein level of RXRA in diabetic group decreased significantly in skeletal muscle tissue. The expression of Rxra in the skeletal muscle and adipose tissue of diabetic gerbils was lower than that in other tissues, but there was no statistical difference between the two tissues. Conclusion the expression of Rxra is low in skeletal muscle and adipose tissue of diabetic gerbils. There was no difference in the expression level among the other four tissues, indicating that the effect of Rxra on diabetes in gerbils was mainly in skeletal muscle and adipose tissue.
【作者单位】：首都医科大学基础医学院;
【基金】：国家科技支撑计划(编号:2015BAI09B01) 国家自然科学基金(编号:31272393和31572348) 北京市自然科学基金(编号:7141002)
【分类号】：R-332;R587.1
【正文快照】： 。根据国际糖尿病联盟(IDF)统计,2015年全球糖尿病患者为4.15亿,预计到2040年糖尿病人数将达到6.42亿[1]。目前,中国糖尿病患病人群已达到1.096亿,并且发病率呈现上升趋势,其中2型糖尿病(T2DM)患者占90%以上[2],2型糖尿病是由遗传因素和环境因素共同作用导致的复杂遗传病,以葡

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