IL-17和Foxp3在小鼠克罗恩病模型中的表达及其意义

发布时间：2018-01-14 06:24

  本文关键词：IL-17和Foxp3在小鼠克罗恩病模型中的表达及其意义　出处：《医学综述》2016年09期 　论文类型：期刊论文

  更多相关文章： 克罗恩病 白细胞介素 叉头状/翅状螺旋蛋白 免疫偏倚

【摘要】：目的观察小鼠克罗恩病模型外周血单核细胞及结肠组织中白细胞介素(IL)17、叉头状/翅状螺旋蛋白3(Foxp3)的表达,探讨其在2,4,6-三硝基苯磺酸(TNBS)诱导的克罗恩病小鼠急性炎症中的作用。方法共40只6~8周龄雌性Bal/c小鼠,采用随机数字表法随机分为两组,20只以TNBS/乙醇溶液灌肠诱导克罗恩病模型(实验组),另外20只小鼠以同等浓度的乙醇氯化钠灌肠(正常对照组)。干预1周后行疾病活动指数(DAI),结肠病理组织学评分(TDI)和结肠大体形态损伤指数(CMDI)评价,以实时荧光定量反转录聚合酶链反应(RT-PCR)检测小鼠外周血单核细胞IL-17mRNA、Foxp3 mRNA的表达,采用免疫印迹法检测小鼠结肠组织IL-17蛋白、Foxp3蛋白的表达。结果与正常对照组相比,实验组的DAI、TDI、CMDI显著升高[DAI(5.41±0.48)比(0.85±0.07),TDI(5.63±0.74)比(0.74±0.08),CMDI(6.88±0.84)比(0.91±0.06),P0.01],外周血单核细胞的IL-17 mRNA显著升高[(1.118±0.145)比(1.000±0.000),P0.01],与其DAI、TDI及CMDI呈正相关(r=0.875,0.935,0.825,P0.01),Foxp3 mRNA显著降低[(0.797±0.271)比(1.000±0.000),P0.01],与其DAI、TDI及CMDI呈负相关(r=-0.885,-0.734,r-0.812,P0.01),结肠组织IL-17蛋白显著上升[(0.631±0.331)比(0.272±0.112),P0.01],与其DAI、TDI及CMDI呈正相关(r=0.765,0.778,0.734,P0.01),Foxp3蛋白显著降低[(0.274±0.112)比(0.592±0212),P0.01],与DAI、TDI及CMDI呈负相关(r=-0.889,-0.809,-0.851,P0.01)。结论在TNBS诱导的结肠炎中,Th17/Treg轴的免疫偏倚是导致克罗恩病发生和发展的重要因素。
[Abstract]:Objective To observe the effect of Crohn's disease model mice peripheral blood mononuclear cells and colon tissue of interleukin 17 (IL), forkhead / winged helix protein 3 (Foxp3) expression on 2,4,6- three trinitrobenzene sulfonic acid (TNBS) in mice induced by acute inflammation in Crohn's disease in rats. Methods a total of 40 only 6~8 week old female Bal/c mice were randomly divided into two groups, 20 rats in TNBS/ ethanol solution enema induced model of Crohn's disease (experimental group), another 20 mice with the same concentration of sodium chloride and ethanol enema (normal control group). The intervention was performed after 1 weeks of disease activity index (DAI). Colon histopathology score (TDI) and colon gross morphological damage index (CMDI) evaluated by real-time fluorescent quantitative reverse transcription polymerase chain reaction (RT-PCR) IL-17mRNA in monocytes in peripheral blood to detect expression of Foxp3, mRNA, the immune blotting detection of colon tissue of mice IL-17 铔嬬櫧,Foxp3铔嬬櫧鐨勮〃杈，

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