高通量测序H6N6亚型禽流感病毒及遗传进化分析

发布时间：2018-01-14 08:11

本文关键词：高通量测序H6N6亚型禽流感病毒及遗传进化分析　出处：《华南预防医学》2016年04期 　论文类型：期刊论文

【摘要】：目的对2013年在广东地区活禽交易市场外环境中分离到的2株H6N6亚型禽流感病毒株进行全基因组测序和遗传进化分析,为广东地区外环境禽流感病毒监控提供实验数据。方法应用Ion Torrent对2份样本[A/Environment/Guangdong/SW-C12382070/2013(H6N6)和A/Environment/Guangdong/SW-C12382099/2013(H6N6)]进行高通量测序,然后采用分子生物学软件对测序结果进行序列分析。结果 BLAST结果显示,2株毒株血凝素蛋白(HA)基因裂解位点附近序列均为P-Q-I-E-T-R-G,仅含有1个碱性氨基酸,是典型的低致病性禽流感病毒特征序列。2株毒株的HA基因与越南分离株A/duck/Nha Trang/60/2013(H6N6)相似性最高,神经氨酸酶(NA)基因与湖南分离株A/chicken/Hunan/S4495/2010(H6N6)相似性最高。全基因组序列遗传进化分析结果显示,2株毒株的8个基因片段均属于欧亚谱系的禽源进化分支,其中HA基因属于ST2853-like分支,NA基因属于ST192-like分支,核蛋白(NP)基因属于HN573-like分支,碱性聚合酶2(PB2)、碱性聚合酶1(PB1)、酸性聚合酶(PA)、基质蛋白(M)和非结构蛋白(NS)基因属于ST339-like分支。结论本研究中的2株H6N6亚型禽流感病毒株为同一亚型不同基因群间基因交换的重组病毒。
[Abstract]:Objective to sequence the whole genome and analyze the genetic evolution of two strains of H6N6 subtype avian influenza virus isolated from live poultry market in Guangdong in 2013. To provide experimental data for monitoring avian influenza virus in Guangdong province. Methods two samples were collected by Ion Torrent. [Ar / Environmental / Guangdong / SW-C12382070 / 2013H6N6). And A / P environment / SW-C12382099 / 2013 / H6N6)] high-throughput sequencing. Then the sequencing results were analyzed by molecular biology software. The results of BLAST showed that. The sequence near the cleavage site of the two strains of hemagglutinin protein (HA) gene was P-Q-I-E-T-R-G, which contained only one basic amino acid. The HA gene of A / Duck / Nha Trang-R / 60 / 2013 H6N6 is the most similar between the HA gene of the typical low pathogenic avian influenza virus characteristic sequence .2 strain and the Vietnamese isolate A / Duck / Nha Trang-R / 60 / 2013 (H6N6). Neuraminidase NAA gene and Hunan isolate A / chicken / Hunan / S 4495 / 2010 H6N6). The genetic evolution analysis of the whole genome sequence showed the highest similarity. The 8 gene fragments of the two strains belong to the avian evolutionary branch of the Eurasian lineage, and the HA gene belongs to the ST2853-like branch and na gene belongs to the ST192-like branch. The nucleoprotein (NPN) gene belongs to the HN573-like branch, alkaline polymerase 2 (Pb2), alkaline polymerase 1 (Pb1) and acidic polymerase (PPA). Matrix protein (M) and nonstructural protein (NSN). Conclusion the two strains of H6N6 subtype avian influenza virus in this study are recombinant viruses with gene exchange among different gene groups of the same subtype.
【作者单位】：广东省疾病预防控制中心;华南农业大学兽医学院;中山大学公共卫生学院;广州市疾病预防控制中心;
【分类号】：R373
【正文快照】： 禽流感病毒(AIV)属于正粘病毒科流感病毒属的A型流感病毒,基因组含8个节段,能引起禽类呼吸道感染或疾病综合征。根据病毒表面血凝素(HA)和神经氨酸酶(NA)抗原性的不同可将流感病毒划分为H1~H18共18个HA亚型和N1~N11共11个NA亚型[1-2],除H17N10和H18N11分离于蝙蝠,目前已知的各

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