四种重要医学媒介蚊虫离子受体基因IR8a和IR25a的特征及分类地位

发布时间：2018-01-15 00:05

  本文关键词：四种重要医学媒介蚊虫离子受体基因IR8a和IR25a的特征及分类地位　出处：《昆虫学报》2017年04期 　论文类型：期刊论文

  更多相关文章： 蚊虫 iGluRs IRs IRa IRa 全基因组鉴定 序列特征 系统发育

【摘要】：【目的】鉴定中华按蚊Anopheles sinensis、冈比亚按蚊Anopheles gambiae、埃及伊蚊Aedes aegypti和致倦库蚊Culex quinquefasciatus 4种重要医学媒介蚊虫的离子受体基因IR8a与IR25a以及代表性的iGluRs基因和IRs基因,研究这些基因的特征及其系统发育关系,确定IR8a与IR25a基因在离子谷氨酸受体基因中的分类地位。【方法】以黑腹果蝇Drosophila melanogaster的iGluRs和IRs基因序列作为询问序列,用BLASTP和BLASTN方法分别搜索中华按蚊、冈比亚按蚊、埃及伊蚊和致倦库蚊的基因组数据库,以鉴定这4个种内的iGluRs和IRs基因序列;用生物信息学方法比较分析鉴定获得的IR8a与IR25a及代表性的iGluRs和IRs基因氨基酸序列的特征;用最大似然法、贝叶斯法和最大简约法构建并讨论这些基因氨基酸序列的系统发育关系;用PAML软件计算这些基因的dN/dS(ω)值并分析其选择压力。【结果】通过对中华按蚊、冈比亚按蚊、埃及伊蚊和致倦库蚊中离子谷氨酸受体基因进行生物信息学鉴定,获得了所有IR8a和IR25a基因,24个代表性的iGluRs基因和30个代表性的IRs基因的序列。特征比较和系统发育关系分析结果表明,在4种蚊虫和黑腹果蝇中IR8a与IR25a基因的氨基酸序列长度与iGluRs的更接近,其平均值远大于其他IRs的;IR25a和IR8a有与传统iGluRs相同的氨基酸末端区域(amino-terminal domain,ATD),虽然IR8a的序列不太保守,而IRs则没有ATD;IR8a与IR25a序列有与传统iGluRs一样保守的配体结合区S1和S2,而IRs没有。所有这些基因的ω值远小于1,意味着这些基因在进化过程中都经历了纯化选择,而IR8a与IR25a的ω值更接近iGluRs。此外,IR8a与IR25a还有与non-NMDA相似的特异性位点,而这些位点在IRs不存在。再者,IR8a和IR25a与non-NMDA聚在一枝,形成一个姐妹群,群内序列间的遗传距离小于本研究中任何其他序列间的距离。根据这些研究结果,我们将IR8a与IR25a分类进入传统iGluRs中的non-NMDA类型,并命名为Putative受体。【讨论】本研究构建了蚊虫iGluRs和IRs基因的信息框架,确定了IR8a与IR25a基因的分类地位,对进一步的功能研究具有重要意义。
[Abstract]:[Objective] identification of Anopheles sinensis Anopheles sinensis, Gambia Anopheles gambiae Aedes mosquito Anopheles, Culex Culex aegypti and quinquefasciatus 4 kinds of important medical vector mosquito ion receptor gene IR8a and IR25a and the representative of the iGluRs gene and IRs gene, phylogenetic relationships of the characteristics and system of these genes, determine the taxonomic status of IR8a and IR25a gene the ionotropic glutamate receptors genes. [method] with iGluRs and IRs gene sequences of Drosophila melanogaster Drosophila melanogaster as query sequences, the search of Anopheles sinensis, respectively using BLASTP and BLASTN methods in Gambia Anopheles, Culex quinquefasciatus and Aedes aegypti genome database, with iGluRs and IRs gene sequences of 4 species in this identification; by comparative analysis of biological information science methods identified with IR25a and IR8a on behalf of iGluRs and the amino acid sequence of IRs gene Characteristics; maximum likelihood, Bayesian and maximum parsimony method is constructed and discussed the relationship between the amino acid sequences of these genes system development; calculation of these genes using dN/dS PAML software (omega) value and analyze the selection pressure. [results] by Anopheles sinensis, Gambia Anopheles, Aedes aegypti and induced by glutamate receptor ion in Culex quinquefasciatus gene bioinformatics identification, access to all the IR8a and IR25a gene, iGluRs gene sequences of 24 representative and 30 representative IRs genes. Comparison of the characteristics and relationship between the phylogenetic analysis results showed that the amino acid sequence of iGluRs and IR8a and IR25a gene in 4 species of mosquitoes and insects in Drosophila melanogaster. Closer, the average value is far greater than other IRs; IR25a and IR8a have the same amino acids with the traditional iGluRs (amino-terminal domain, ATD terminal region), although the IR8a sequence is not too conservative, but not IRs, ATD; I R8a and IR25a have the same sequence with traditional iGluRs ligand binding region conserved S1 and S2, but not IRs. All of these gene values far less than 1, means that these genes in the evolutionary process have experienced negative selection, and the value of IR8a and omega IR25a is close to iGluRs. IR8a and IR25a and in addition, similar to non-NMDA the specific sites, and these sites do not exist in IRs. Furthermore, IR8a and IR25a and non-NMDA were clustered in the same branch, formed a sister group, the genetic distance between sequences within the group is less than the distance between any other sequences in this study. According to these results, we will IR8a and IR25a into the traditional iGluRs classification the type of non-NMDA, and named as Putative receptor. [Conclusion] this study constructs the information framework mosquito iGluRs and IRs genes, determine the taxonomic status of IR8a and IR25a gene, has important significance for the further research on the function.

【作者单位】： 重庆师范大学昆虫与分子生物学研究所媒介昆虫重庆市重点实验室;
【基金】：“两江学者”计划专项经费 国家自然科学基金项目(31672363,31372265) 国际原子能机构CRP项目(18268) 国家科技基础性工作专项重点项目(2015FY210300)
【分类号】：R384.1
【正文快照】： 离子谷氨酸受体(ionotropic glutamate receptors,i Glu Rs)是真核生物和原核生物中一类保守的配体结合离子通道,在脊椎动物神经系统突触间的信号传递中作为兴奋的神经递质谷氨酸的受体,通过调节阳离子流跨质膜来应答细胞外谷氨酸和相关配体的结合,细胞可以利用其来调节内部生

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