国产水痘减毒活疫苗关键基因的序列分析和比较

发布时间：2018-01-15 03:19

本文关键词：国产水痘减毒活疫苗关键基因的序列分析和比较　出处：《中国医学科学院学报》2017年05期 　论文类型：期刊论文

更多相关文章： 水痘疫苗 基因变异 ORF ORF ORF

【摘要】：目的从基因水平分析国产水痘减毒活疫苗的安全性和一致性。方法对国内4家企业生产的水痘减毒活疫苗的关键基因(ORF38、ORF54和ORF62)进行PCR扩增和序列测定,并与Genbank公布的Dumas、P-Oka、V-Oka及国外上市疫苗Varilrix(GSK)、Varivax(Merck)的序列进行比对。结果 4家国产水痘减毒活疫苗的ORF38和ORF54区基因序列一致,并且同V-Oka、Varilrix完全一致,与Varivax(Merck)有1处差异。4家国产水痘减毒活疫苗的ORF62区基因序列基本一致,与V-Oka、Varilrix(GSK)和Varivax(Merck)均存在个别核苷酸差异。结论 4家国产水痘减毒活疫苗的ORF38、ORF54和ORF62区基因序列基本一致,稳定性较好,与V-Oka、Varilrix(GSK)和Varivax(Merck)存在较小差异,没有引入新的突变位点。
[Abstract]:Objective to analyze the safety and consistency of domestic Live Attenuated Vaccine Against Varicella from the gene level. Key genes of the 4 domestic manufacturers Live Attenuated Vaccine Against Varicella (ORF38, ORF54 and ORF62) were determined by PCR amplification and sequence, and with the release of Genbank Dumas, P-Oka, V-Oka and Varilrix (GSK) vaccine market abroad, Varivax (Merck). Sequence alignment. The genes ORF38 and ORF54 sequences of 4 domestic Live Attenuated Vaccine Against Varicella, and with V-Oka, Varilrix and Varivax completely consistent, (Merck) ORF62 gene sequences of 1 different.4 domestic Live Attenuated Vaccine Against Varicella consistent with V-Oka, Varilrix (GSK) and Varivax (Merck) are individual nucleotide differences conclusion. 4 domestic Live Attenuated Vaccine Against Varicella ORF38, ORF54 gene sequence and ORF62 are basically the same, good stability, and V-Oka, Varilrix (GSK) and Varivax (Merck) there were small No new mutation site was introduced.

【作者单位】：中国食品药品检定研究院生物制品检定所呼吸道病毒疫苗室;
【分类号】：R392-33
【正文快照】： 水痘是由水痘-带状疱疹病毒引起的一种高度传染病,常见于儿童。接种水痘疫苗是预防和控制水痘发生的最有效手段。Oka水痘病毒株(V-Oka)是世界卫生组织认可的唯一可用于水痘疫苗生产的减毒株,也是我国水痘减毒活疫苗的生产用毒株[1-2]。V-Oka是将典型水痘患者疱疹液中分离得到

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