血脂平胶囊对高血脂模型金黄地鼠血脂水平的影响及机制研究

发布时间：2018-01-15 11:37

本文关键词：血脂平胶囊对高血脂模型金黄地鼠血脂水平的影响及机制研究　出处：《中国药房》2017年16期 　论文类型：期刊论文

【摘要】：目的:研究血脂平胶囊对高血脂模型金黄地鼠血脂水平的影响及其可能机制。方法:将金黄地鼠随机分为正常对照组、模型组、阿托伐他汀组(3 mg/kg)、联用组(血脂平胶囊1.3 g/kg+阿托伐他汀1.5 mg/kg)和血脂平胶囊低、中、高剂量组(血脂平胶囊1.3、2.6、5.2 g/kg),每组10只。正常对照组地鼠给予普通饲料,其他组地鼠给予高脂饲料建立高血脂模型,2周后同时ig给予相应药物,正常对照组和模型组地鼠ig等体积的蒸馏水,每日1次,连续给药4周。末次给药后,检测各组地鼠血脂指标[总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFA)],肝组织中脂质代谢相关基因(PPAR-α、CYP7A1、ACOX、SREBP-1c、ACC1)m RNA及蛋白表达。结果:与正常对照组比较,模型组地鼠血清TC、TG、LDL-C、FFA含量增加,HDL-C含量降低,PPAR-α、CYP7A1、ACOX m RNA及蛋白表达减弱,SREBP-1c、ACC1 m RNA及蛋白表达增强(P0.05)。与模型组比较,血脂平胶囊高剂量组、阿托伐他汀组和联用组地鼠上述指标均明显改善(血脂平高剂量组HDL-C除外)(P0.05);血脂平胶囊中剂量组地鼠TC、TG、FFA含量和PPAR-α、CYP7A1、ACOX、ACC1 m RNA及CYP7A1、SREBP-1c、ACC1蛋白表达均明显改善(P0.05);血脂平胶囊低剂量组地鼠TC、TG含量和PPAR-αm RNA及CYP7A1、SREBP-1c蛋白表达均明显改善(P0.05)。结论:血脂平胶囊具有降血脂作用,其机制可能与激活PPAR-α和抑制SREBP-1c信号通路有关。
[Abstract]:Objective: to study the effect and possible mechanism of Shuiping capsule on hyperlipidemia model golden hamster. Methods: Golden hamsters were randomly divided into normal control group and model group. Atorvastatin group (3 mg / kg), combined group (1.3 g / kg Atorvastatin 1.5 mg / kg) and lipids capsule (1.5 mg / kg) were low and medium. 10 rats in each group were given normal diet in normal control group and hyperlipidemia model was established in other groups. After 2 weeks of administration, the rats in the normal control group and the model group were given the same volume of distilled water intravenously, once a day for 4 weeks. After the last administration, the blood lipids were measured in each group. [Total cholesterol (TC), triglyceride (TGN), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), free fatty acid (FFA). Results: the expression of PPAR- 伪 CYP7A1ACOXP-1cACC1m RNA and protein in liver tissue was compared with that in normal control group. In the model group, the serum TCG-TGG LDL-CfFA increased the content of HDL-C and decreased the expression of PPAR- 伪 -CYP7A1ACox m RNA and protein. The expression of ACC1 m RNA and protein in SREBP-1cACC1 m were increased (P 0.05). Compared with the model group, the high dose group of Shuiping capsule. The above indexes were significantly improved in both atorvastatin group and combination group (except for HDL-C with high dose of lipids). In the middle dose group, the content of TCN TGG FFA, PPAR- 伪 CYP7A1ACOX1 m RNA and CYP7A1 + SREBP-1c were measured. The expression of ACC1 protein significantly improved P0.05A; In the low dose group, the content of TG, PPAR- 伪 m RNA and CYP7A1 were measured. The expression of SREBP-1c protein was significantly improved by P0.050.Conclusion: Shuiping capsule has the effect of lowering blood lipids. The mechanism may be related to activation of PPAR- 伪 and inhibition of SREBP-1c signaling pathway.
【作者单位】：哈尔滨商业大学生命科学与环境科学研究中心;中国医学科学院北京协和医学院药用植物研究所;
【分类号】：R285.5;R-332
【正文快照】： *硕士研究生。研究方向:心血管药理。电话:010-51873226。E-mail:qshen666@126.com由于不良生活方式的影响,在中国,高脂血症(Hy-perlipidemia,HLP)已成为一种常见的代谢性疾病。该疾病特点为脂代谢异常,包括总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)异常升高和高密度脂

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