吸虫CD59样蛋白质的生物信息学分析

发布时间：2018-01-15 22:01

本文关键词：吸虫CD59样蛋白质的生物信息学分析　出处：《中国病原生物学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的对在寄生虫中新发现的CD59样蛋白序列进行生物信息学分析,为研究其功能奠定基础。方法应用生物信息学方法,从不同的数据库中查找6种吸虫(肝片吸虫、大片吸虫、日本血吸虫、曼氏血吸虫、华支睾吸虫、麝猫后睾吸虫)的CD59样蛋白序列,应用生物信息学软件对获得的序列进行信号肽和GPI锚定蛋白的预测和分析,应用Clustlw与人CD59序列进行比对分析,并应用Mrbayes构建进化树。结果 6种吸虫分别存在5-11种不同的CD59样蛋白,大小98~137aa,富含半胱氨酸,均有LY6/CD59蛋白质家族的保守膜体"CCXXXXCN"。与人CD59比对,在10个半胱氨酸中有8个相互匹配。根据6种吸虫中获得的46条CD59样蛋白的氨基酸序列构建系统进化树,可将吸虫分为6个不同的大群,其中有3个群均包含裂体科、片形科和后睾科吸虫序列,另外3个为裂体科、片形科和后睾科吸虫各自独立成群。结论吸虫中存在一类CD59样蛋白家族,这类蛋白质与人CD59有类似的结构,进化分析提示该类蛋白质功能具有多样性。
[Abstract]:Objective to study the function of CD59 like protein in parasites by bioinformatics analysis. Methods Bioinformatics method was used. The CD59 like protein sequences of 6 kinds of trematodes (clonorchiasis, clonorchiasis, Schistosoma japonicum, Schistosoma mansoni, clonorchiasis sinensis, musk deer cat clonorchiasis) were found from different databases. The signal peptides and GPI anchoring proteins were predicted and analyzed by bioinformatics software, and Clustlw was compared with human CD59 sequences. Mrbayes was used to construct an evolutionary tree. Results there were 5-11 different CD59 like proteins in the 6 species of trematodes, 98137aa in size, rich in cysteine. The conserved membrane "CCXXXXCN" of LY6/CD59 protein family was compared with human CD59. The phylogenetic tree was constructed according to the amino acid sequences of 46 CD59 like proteins obtained from 6 trematodes. The trematodes can be divided into 6 different groups. Three of them contained the sequences of clonorchidae, laminae and clonorchidae, and the other three were cleft families, laminae and posterior clonorchidae. Conclusion there is a family of CD59 like proteins in trematodes. This kind of protein has similar structure to human CD59, and evolution analysis indicates that this kind of protein has a variety of functions.
【作者单位】：广西壮族自治区疾病预防控制中心寄生虫病防制所;
【基金】：广西壮族自治区疾病预防控制中心青年基金(201501)
【分类号】：Q811.4;R383.2
【正文快照】： ***CD59为约20ku的小蛋白,主要分布于人和哺乳动物的器官和组织中。其主要功能是调节补体的活化,通过非特异性的与C8和C9耦合,抑制膜攻击复合物(membrane attack complex,MAC)的形成,从而保护机体不受MAC的攻击[1]。研究发现CD59在免疫调控和抗癌过程中起重要作用并参与调控T

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