碳纳米管对树突状细胞成熟和T细胞分化作用研究

发布时间：2018-01-15 23:11

本文关键词：碳纳米管对树突状细胞成熟和T细胞分化作用研究　出处：《中国生物医学工程学报》2017年04期 　论文类型：期刊论文

【摘要】：Th分化在免疫应答过程中起着关键性的作用,主要由树突状细胞来调控。碳纳米管是由碳元素组成的空心管状纳米材料,可作为抗原载体。碳纳米管对树突状细胞和Th分化的影响是决定其免疫响应的关键因素。分析氧化多壁碳纳米管(CNT)对小鼠骨髓来源的树突状细胞(BMDCs)功能的影响,以及对过敏抗原多肽OVA323-339的Th1和Th2免疫反应的影响。实验结果显示,BMDCs大量吞噬CNT,但对其细胞表面活化标志分子CD86、MHCII以及细胞因子TNF-α表达没有明显变化。CNT/抗原复合物可促进BMDCs表面MHCII的表达和CD8+T细胞增殖,增殖细胞的比例由28.7%分别增加到40.6%、41.3%和29.6%。Th2型细胞因子IL-4、IL-13和IL-10的表达和CD4+T的增殖受到抑制,增殖细胞的比例由54.4%减少到38.9%、46.6%和39.8%。研究结果提示,CNT不影响DCs的成熟和活化,但CNT可以影响过敏抗原的Th分化平衡,可抑制Th2型细胞因子的表达,促进Th1型免疫响应。
[Abstract]:Th differentiation plays a key role in the process of immune response, mainly by dendritic cells to regulate. Carbon nanotubes are hollow tubular nano materials composed of carbon, can be used as antigen carrier. Effect of carbon nanotubes on dendritic cells and the differentiation of Th is the key factor in immune response. Analysis of oxidized multi walled carbon nanotubes (CNT) on murine bone marrow derived dendritic cells (BMDCs) function and effect, effect of Th1 and Th2 on the immune response of allergic antigen peptide OVA323-339. The experimental results show that the BMDCs swallow CNT, but the cell surface activation markers CD86, MHCII and the expression of cytokines TNF- antigen of.CNT/ did not change significantly. The complex can promote the expression of MHCII on the surface of BMDCs and CD8+T cell proliferation, cell proliferation ratio was increased to 40.6% from 28.7%, 41.3% and 29.6%.Th2 type cytokines IL-4, IL-13 and IL-10 The expression of CD4+T and proliferation was inhibited, cell proliferation ratio decreased from 54.4% to 38.9%, the 46.6% and 39.8%. studies suggest that CNT does not affect DCs maturation and activation, but CNT can affect the balance of the differentiation of Th allergic antigen, can inhibit the expression of Th2 type cytokines promote Th1 type immune response.

【作者单位】：中国医学科学院基础医学研究所北京协和医学院基础学院;中国生物医学工程学会;
【基金】：北京市自然基金(7162128) 协和青年基金(3332016136)
【分类号】：R392
【正文快照】： 引言碳纳米管具有典型的纳米结构和高比表面积,可以与生物大分子结合,易透过细胞膜被多种细胞摄取,可以作为生物分子的高效载体[1-5]。有研究将碳纳米管与手足口病多肽抗原结合,发现与传统的铝佐剂相比,碳纳米管引起了更强的特异性抗体产生[6],可显著地促进半抗原特异性抗体产

【相似文献】