补肾填精方对快速老化模型小鼠活化的T细胞转录核因子蛋白表达的影响

发布时间：2018-01-16 05:18

本文关键词：补肾填精方对快速老化模型小鼠活化的T细胞转录核因子蛋白表达的影响　出处：《时珍国医国药》2017年09期 　论文类型：期刊论文

【摘要】：目的观察补肾填精方左归丸对快速老化模型小鼠骨组织破骨细胞功能的影响,探讨其作用机制是否与干预活化的T细胞转录核因子蛋白表达有关。方法取SAMR1同源对照组小鼠8只为对照组(SAMR1),取SAMP6衰老模型小鼠16只,随机分为模型组(SAMP6)、左归丸组(ZGP),每组8只。灌胃给药12周后收集各组小鼠股骨组织,采用HE染色法观察股骨组织形态,采用TRAP染色法观察股骨组织破骨细胞数,采用Western-blot法检测股骨组织NFAT2蛋白表达水平。结果与SAMR1比较,SAMP6股骨组织形态明显改变、破骨细胞数增多、NFAT2蛋白表达显著升高;与SAMP6比较,补肾填精方能够部分改善模型小鼠的股骨组织形态、减少破骨细胞数、下调NFAT2蛋白表达。结论补肾填精方左归丸对快速老化模型小鼠骨质疏松具有一定的防治作用,其作用机制可能与其通过干预NFAT2蛋白表达调节破骨细胞功能有关。
[Abstract]:Objective To observe the effect of Bushentianjing Zuogui Pill on bone tissue rapid aging model mice osteoclast function expression, to explore the mechanism and intervention of activated T cell nuclear factor protein. Methods SAMR1 homologous control group of 8 mice as control group (SAMR1), 16 SAMP6 of aging model mice, were randomly divided into model group (SAMP6), Zuoguiwan group (ZGP), 8 rats in each group. Collect femoral tissues of mice by gavage for 12 weeks, HE was used to observe the femoral morphology staining, TRAP staining was used to observe the femoral tissue osteoclast number, the level of Western-blot was used to detect NFAT2 protein of femur tissue expression. Results: compared with SAMR1, SAMP6 femoral morphology changed obviously, the number of osteoclasts increased, NFAT2 protein expression was significantly increased; compared with SAMP6, form the femur of Bushentianjing can partly improve mice, reduce bone resorption Conclusion: Bushen Tianjing Fang zuigu pill has a certain preventive and therapeutic effect on osteoporosis in fast aging mice, and its mechanism may be related to its regulation of osteoclast function through intervening the expression of NFAT2 protein. NFAT2

【作者单位】：辽宁中医药大学;
【基金】：国家自然科学基金(No.81503488) 中国博士后科学基金(No.2016T90229)
【分类号】：R285.5;R-332
【正文快照】： 随着全球人口老龄化,老年性骨质疏松发病率逐渐上升,已经成为被广泛关注的社会健康问题。老年性骨质疏松症(seni-leosteoporosis,SOP)是生物衰老在骨骼方面的表现,是一种代谢性骨病,其发生与年龄有关[1]。补肾壮骨方长期服用能明显提高和稳定SOP患者骨矿含量、骨密度水平,降低

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