G15对发情周期小鼠卵巢表皮生长因子受体表达的影响

发布时间：2018-01-16 06:08

本文关键词：G15对发情周期小鼠卵巢表皮生长因子受体表达的影响　出处：《西北农林科技大学学报(自然科学版)》2017年02期 　论文类型：期刊论文

【摘要】：【目的】研究G蛋白偶联雌激素受体(G-protein-coupled estrogen receptor,GPER)对发情周期小鼠卵巢表皮生长因子受体(Epidermal growth factor receptor,EGFR)表达的影响。【方法】间情期小鼠腹腔注射GPER抑制剂G15,按注射剂量0.3,1.5和7.5nmol/只设3个注射组,另设腹腔注射等体积生理盐水的对照组;药物注射后,依次取发情前期、发情期、发情后期和间情期小鼠的卵巢,用免疫组织化学SP法和RT-PCR法分别检测各组小鼠发情周期不同阶段卵巢EGFR及其mRNA的表达。【结果】EGFR免疫组化阳性产物主要分布于卵巢的各级卵泡中,阳性染色随卵泡发育而逐渐增强,其中颗粒细胞越靠近卵母细胞染色越强,发情周期卵巢EGFR相对表达量表现为发情期发情前期间情期发情后期。小鼠注射G15后,各情期卵巢中EGFR的表达呈G15剂量依赖性减弱,除发情后期外,均极显著下降(P0.01);1.5和7.5nmol/只G15注射组各情期EGFR的相对表达量无显著差异(P0.05),但均极显著低于0.3nmol/只G15注射组(P0.01)。EGFR mRNA的变化规律与EGFR相对表达量的变化趋势完全一致。【结论】G15可在一定程度上通过GPER下调发情周期小鼠卵巢中EGFR的表达,从而影响发情周期卵巢的功能。
[Abstract]:[objective] to study G-protein-coupled estrogen receptor (G-protein-coupled estrogen receptor). GPR) on epidermal growth factor receptor of ovaries of estrous cycle mice. The effect of EGFR on the expression of EGFR. [methods] mice were injected intraperitoneally with GPER inhibitor G15 at the doses of 0.3nmol and 7.5nmol.There were only three injection groups. The control group was injected intraperitoneally with normal saline of the same volume. After drug injection, the ovaries of mice in preestrus, estrus, estrus and interoestrus were taken in turn. Immunohistochemical SP method and RT-PCR method were used to detect the expression of EGFR and mRNA in ovary of each group of mice at different stages of estrous cycle. [results] Immunohistochemical positive products of EGFR were mainly distributed. In follicles at all levels of the ovary. Positive staining gradually increased with follicle development, in which granulosa cells closer to the oocyte staining was stronger. The relative expression of EGFR in the ovary of estrous cycle was the late estrous period before estrus. The expression of EGFR was decreased in a dose-dependent manner after the mice were injected with G15. Except for the late estrus, the P0.01D decreased significantly. There was no significant difference in the relative expression of EGFR between 1.5 and 7.5 nmol / mouse G15 injection group (P 0.05). But both of them were significantly lower than 0.3nmol / mouse G15 injection group (P 0.01). .EGFR. The change of mRNA was completely consistent with the trend of relative expression of EGFR. [conclusion] G15 can down-regulate the expression of EGFR in the ovary of estrous cycle mice to some extent through GPER. Thus affect the ovarium function of estrous cycle.
【作者单位】：西北农林科技大学动物医学院;
【基金】：国家林业局科研项目“野生动物疫源疫病监测及预警系统维护”(K313021501)
【分类号】：R339.2
【正文快照】： 作为哺乳动物卵巢分泌的一种重要的类固醇激素,雌激素与雌激素受体(estrogen receptor,ER)结合通过基因组效应调节雌性动物卵巢的生长发育和正常生殖生理功能。研究发现,雌激素还能通过细胞膜表面的G蛋白偶联雌激素受体(G-protein-cou-pled estrogen receptor,GPER)介导的快速

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