四氯化碳致小鼠急性肝损伤模型的优化及痰热清注射液的保肝作用

发布时间：2018-01-16 07:02

本文关键词：四氯化碳致小鼠急性肝损伤模型的优化及痰热清注射液的保肝作用　出处：《中国医药工业杂志》2017年02期 　论文类型：期刊论文

【摘要】：优化了四氯化碳致小鼠急性肝损伤模型的造模条件,并评价痰热清注射液的保肝作用。分别考察了四氯化碳造模时小鼠性别、剂量和时间以及阳性对照药对小鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的影响,以确定优化造模条件。通过ALT和AST指标和肝组织病理形态学变化来评价痰热清注射液对四氯化碳致小鼠急性肝损伤模型的保护作用。结果表明,雄性小鼠、剂量16 mg/kg、造模时间13 h,可作为四氯化碳致小鼠急性肝损伤模型的优化条件,联苯双酯可选为阳性对照药。痰热清注射液对四氯化碳致小鼠急性肝损伤模型有明显的保护作用。
[Abstract]:Optimization of the mouse model of acute liver injury induced by carbon tetrachloride model conditions, and to evaluate the protective effect of Tanreqing injection were investigated. Carbon tetrachloride model mice sex, transaminase dose and time and the positive control drug on mice serum alanine (ALT) and aspartate aminotransferase (AST) effect, in order to determine the optimal model condition. To evaluate the protective effect of Tanreqing Injection on acute hepatic injury in mice induced by CCl4 model by pathological morphological changes of ALT and AST index and liver tissue. The results showed that male mice, a dose of 16 mg/kg, 13 h when modeling, optimization conditions can be used as carbon tetrachloride induced acute liver injury in mice model, bifendate is optional the positive control drug. Tanreqing injection has obvious protective effect on CCl4 induced acute liver injury model in mice.

【作者单位】：上海市食品药品检验所;上海中医药大学;
【基金】：上海市食品药品检验所所级课题(2015-SKT-13)给予资助
【分类号】：R285.5;R-332
【正文快照】： 方中所含药材为黄芩、熊胆粉、山羊角、金银花、连翘等,具有清热、解毒、化痰、解惊之功效。现代药理试验研究表明,痰热清注射液具有抗菌、镇咳、化痰、平喘、解惊作用。临床主要用于治疗成人急性肺炎、慢性支气管炎急性发作和慢性阻塞性肺病急性感染,能有效缓解发热、咳嗽、

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