IRE1信号通路在热诱导睾丸生殖细胞凋亡中的作用

发布时间：2018-01-16 08:05

本文关键词：IRE1信号通路在热诱导睾丸生殖细胞凋亡中的作用　出处：《毒理学杂志》2016年02期 　论文类型：期刊论文

更多相关文章： 热应激 睾丸 生殖细胞 凋亡 肌醇必需酶

【摘要】：目的探讨内质网应激IRE1信号通路在急性热应激诱导睾丸生殖细胞凋亡中的作用。方法 24只成年ICR雄性小鼠,随机分为4组。将热处理组小鼠身体的下1/3部分(包括阴囊、后腿和尾巴)浸于43℃恒温水浴中15 min,于热处理后0.5、2和6 h取睾丸组织。将对照组小鼠下腹部浸入22℃水浴15 min,6 h后取睾丸组。采用TUNEL法检测睾丸组织生殖细胞凋亡;用半定量RT-PCR技术检测u XBP-1/s XBP-1 mRNA表达;用Western blot检测睾丸总蛋白p-JNK表达。结果单次热应激能够明显诱导小鼠睾丸生殖细胞凋亡。与对照组相比,热处理能够明显降低睾丸组织u XBP-1/s XBP-1 mRNA的比值,以热处理后0.5和2 h最为显著,而随着时间的延长,又逐渐恢复正常。热应激明显诱导睾丸JNK(另一个IRE1的下游分子)磷酸化水平。结论单次热处理能激活睾丸组织内质网应激IRE1通路,在早期可能激活IRE1-XBP-1通路保护睾丸组织对抗热应激,而后期可能通过激活IRE1-JNK通路诱导睾丸生殖细胞凋亡。
[Abstract]:Objective to investigate the endoplasmic reticulum stress signaling pathway of IRE1 induced testicular germ cell apoptosis in acute heat stress. Methods 24 adult male ICR mice were randomly divided into 4 groups. The heat treatment under part 1/3 mice body (including the scrotum, hind legs and tail) immersed in a constant temperature of 43 DEG C water bath in 15 min. In 0.5,2 after heat treatment and 6 h testicular tissue. The mice of control group under the belly in 22 DEG C water bath for 15 min and 6 h testis after group. TUNEL method was used to detect the apoptosis of germ cells in testis tissue; the expression of u was detected by semi quantitative mRNA XBP-1/s XBP-1 RT-PCR Western blot detection technology; expression of testis total protein p-JNK. The results of single heat stress can induce testicular germ cell apoptosis in mice. Compared with the control group, heat treatment can significantly reduce the ratio of u XBP-1/s XBP-1 mRNA in testis, with heat treatment after 0.5 and 2 h were the most significant, and with the extension of time, And gradually returned to normal. The heat stress induced testicular JNK (downstream molecules of another IRE1) phosphorylation level. Conclusion single heat treatment can activate the testicular tissue of the endoplasmic reticulum stress pathway of IRE1 in early may activate IRE1-XBP-1 pathway in testicular tissue protection against heat stress, while the latter may activate the IRE1-JNK pathway to induce apoptosis of testis germ cells.

【作者单位】：安徽医科大学公共卫生学院;安徽医科大学第二临床医学院;
【基金】：国家自然科学基金(31571557) 安徽医科大学“青年拔尖人才支持计划” 2015年安徽医科大学省级大学生创新创业训练项目(201510366082) 安徽医科大学“5+3”一体化临床医学专业“早期接触科研”训练计划
【分类号】：R339.21
【正文快照】： 近年来,关于高温与男性生殖的关系已经成为生殖医学领域的研究热点。以往的研究发现洗桑拿浴或高温作业下的成年男子生育力明显降低,一些研究已证实阴囊温度略有增加就能够干扰精子发生甚至导致不育[1-4]。睾丸生殖细胞凋亡是阴囊过热引起男(雄)性不育的原因之一,但热处理诱导

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