恐惧记忆的形成对大鼠内侧前额叶皮层局部场电位的影响

发布时间：2018-01-16 10:39

本文关键词：恐惧记忆的形成对大鼠内侧前额叶皮层局部场电位的影响　出处：《中国生物医学工程学报》2017年01期 　论文类型：期刊论文

【摘要】：根据大鼠恐惧记忆形成前后不同时间段局部场电位的变化特征,研究恐惧记忆的形成对内侧前额叶皮层局部场电位的影响。选取健康成年雄性SD大鼠8只,利用脑立体定位仪将微丝电极植入到大鼠的内侧前额叶皮层;手术恢复后1周,在清醒状态下,通过穿梭恐惧记忆训练诱导出大鼠的恐惧记忆。借助Cerebus神经信号记录系统,记录大鼠在清醒状态下恐惧记忆形成前和恐惧记忆形成后6 h及1、2、3、4、5、6 d的内侧前额叶皮层的局部场电位信号,并采用Neuroexplorer软件分析局部场电位信号。以功率谱密度和功率百分比参数为指标,对恐惧记忆形成前后的总功率和不同时间状态下的各频段(δ:1~3 Hz,θ:4~7 Hz,α:8~13 Hz,β:14~30 Hz,γ:31~100 Hz)功率百分比进行方差分析。实验结果显示,恐惧记忆形成后6 h,θ波和β波的功率百分比较恐惧记忆形成前具有显著性差异(P=0.017,P=0.017),θ波较恐惧记忆形成前功率百分比增加,β波较恐惧记忆形成前功率百分比降低;另外,恐惧记忆形成后第1、2 d,δ波的功率百分比较恐惧记忆形成前具有显著性差异(P=0.03,P=0.049),γ波的功率百分比较恐惧记忆形成前具有显著性差异(P=0.013,P=0.019)。研究表明,恐惧记忆的形成对内侧前额叶皮层神经信息处理的模式产生显著性的影响。
[Abstract]:According to the changes of local field potential before and after the formation of fear memory in rats, the effects of fear memory formation on the local field potential of medial prefrontal cortex were studied. Eight adult male SD rats were selected. The microfilament electrode was implanted into the medial prefrontal cortex of the rat by using the brain stereotactic locator. One week after recovery, fear memory was induced by shuttling fear memory training in awake state. Cerebus neural signal recording system was used to induce fear memory in rats. The local field potential signals of the medial prefrontal cortex were recorded before and 6 hours after the formation of fear memory in the conscious state and in the medial prefrontal cortex 6 hours after the formation of fear memory. The local field potential signal is analyzed by Neuroexplorer software. The parameters of power spectrum density and power percentage are taken as the index. The total power before and after the formation of fear memory and the frequency bands (未: 1 / 3 Hz, 胃: 4 ~ 7 Hz, 伪: 813 Hz, 尾: 1430 Hz) before and after the formation of fear memory were studied. The power percentage of 纬: 31 ~ 100 Hz) was analyzed by ANOVA. The experimental results showed that the fear memory was formed 6 hours after the formation of fear memory. The power percentage of 胃 wave and 尾 wave was significantly higher than that before fear memory formation, and the power percentage of 胃 wave was higher than that before fear memory formation. The power percentage of 尾 wave was lower than that before fear memory formation. In addition, the power of 未 wave was significantly higher than that before the formation of fear memory at 1 and 2 days after the formation of fear memory (P < 0.03 / P ~ (0.049)). The power percentage of 纬 wave was significantly different from that before the formation of fear memory. The formation of fear memory has a significant effect on the pattern of neural information processing in medial prefrontal cortex.
【作者单位】：上海理工大学医疗器械与食品学院;
【基金】：国家自然科学基金(11179015,51173108) 上海理工大学科技发展项目(16KJFZ107)
【分类号】：R338
【正文快照】： 引言植入到大脑皮层内的微电极可以采集到微电极周围由树突活动产生的局部场电位神经电信号,它反映来自神经元网络局部神经核团的活动状态。局部场电位(local field potential,LFP)是多个神经元兴奋性或抑制性突触后电位在记录点的总和,是在慢尺度时间窗口中产生的慢电位[1]。

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