植物乳杆菌微胶囊制备技术及其消化道耐受性研究

发布时间：2018-01-17 22:04

本文关键词：植物乳杆菌微胶囊制备技术及其消化道耐受性研究　出处：《南昌大学》2012年硕士论文　论文类型：学位论文

【摘要】：作为一类与宿主互利共生、有利于宿主健康的生理细菌,益生菌能够通过改善肠道微生态平衡而促进人体健康。益生菌制剂就是利用益生菌能够调节肠道菌群生态平衡,对宿主起有益作用的特点而制成的活性微生态制剂,乳杆菌是常用的益生菌菌种。但是消化道中低pH值的胃酸和胆汁会给活性益生菌造成很大的杀灭,极大地影响了益生菌在肠道中定植并发挥生理功能。本研究采用微胶囊化技术与真空干燥技术相结合的方法,以海藻酸钠和脱脂乳作为混合壁材,将植物乳杆菌和冻干保护剂一起作为芯材,对益生菌进行包埋,并进行真空干燥处理以减少胶囊中的水分,延长益生菌制剂的贮存时间,使益生菌能够顺利通过胃液到达肠道中发挥益生作用。本试验研究了挤压法制备肠溶性植物乳杆菌微胶囊的工艺,通过优化设计确定了最佳制备条件,同时优化了冷冻干燥过程中复合保护剂的成分。此外,还采用改良的两步真空干燥法替代了传统的真空冷冻干燥法,提高了冻干胶囊中的菌体存活率。并在此基础上通过模拟胃肠液处理、贮存稳定性处理等应用性研究,对胶囊产品进行了性能评估。研究内容和结果如下： 1、利用海藻酸钠形成的胶囊具有肠溶而胃不溶的特点以及乳清蛋白在低pH值条件且有胃蛋白酶存在的情况下依然能够保持结构完整的特性,将海藻酸钠和脱脂乳作为植物乳杆菌微胶囊化的混合壁材。通过响应面优化设计,确定挤压法制微胶囊的最佳工艺条件为：海藻酸钠质量分数2.68%,氯化钙浓度0.20mol/L,脱脂乳质量分数4.17%。按此工艺制备的微胶囊在胃液中处理3h后,微胶囊中的活菌数可达8.79×109CFU/g。 2、在单因素试验的基础上筛选得到4种在冷冻干燥过程中对植物乳杆菌保护效果较好的冻干保护剂,通过正交试验得到最佳复合冻干保护剂配方为：海藻糖8%、乳糖8%、维生素C0.2%、甘油3%。用此配方冻干微胶囊,可使菌体存活率达到87.2%。 3、利用改良的两步真空干燥法即真空恒温干燥和真空冷冻干燥相结合的方法,对微胶囊进行干燥处理。不仅可以缩短真空冷冻干燥的时间,而且可以使冻干胶囊中的活菌细胞存活率提高至90.3%。 4、按照优化工艺制备获得的植物乳杆菌微胶囊在人工胃液中处理3h后,活菌菌体存活率可以达到80%左右,说明经过微胶囊化处理,益生菌菌体对胃酸环境的抵抗力得到增强。 5、按照优化工艺制备获得的植物乳杆菌胶囊在人工肠液中处理45min后,溶液的透光率降至最低值,且看不到固体颗粒,说明微胶囊已基本崩解完全,达到中华人民共和国药典2005年版标准的要求。由此可以判断,按照本实验优化工艺制备的植物乳杆菌微胶囊具备良好的肠溶性。 6、冻干胶囊在-20℃条件下贮存60d后,微胶囊中菌体存活率接近70%。在2℃条件下贮存60d后,仍然有接近60%的存活率。说明采用本实验优化工艺获得的微胶囊化方法可以有效提高植物乳杆菌在低温下的贮存稳定性。
[Abstract]:As a kind of physiological bacteria that is beneficial to the host's health, probiotics can promote the health of the human body by improving the intestinal microecological balance.
Is the use of probiotics probiotics can regulate the ecological balance of intestinal flora, made the activity of probiotics on host characteristics beneficial role of Lactobacillus probiotics is commonly used. But the digestive tract and bile acid of low pH value can cause much to kill activity of probiotics, greatly affected the probiotics in intestinal tract and play a physiological function.
This research adopts the method of microencapsulation technology and vacuum drying technology combined with sodium alginate, and skim milk as a mixture of wall material, Lactobacillus plantarum and protectants as the core material, the embedding of probiotics, and vacuum drying treatment to reduce the moisture in the capsule, prolong the storage time of probiotics so, probiotics can arrive smoothly through the gastric juice probiotic in the gut.
The experimental study on the preparation of the enteric microcapsule of Lactobacillus plantarum by extrusion, by optimizing the design to determine the optimum preparation conditions, and optimize the composition of composite protective agent during freeze drying process. In addition, the two step modified vacuum drying method instead of the traditional vacuum freeze drying method, improved freeze drying capsule in the survival rate of bacteria. And on the basis of simulated gastrointestinal treatment, storage stability and application research, to evaluate the performance of capsule products. The research contents and results are as follows:
1, the use of sodium alginate with enteric capsule formation and characteristics of gastric insoluble and whey protein in low pH value and is still able to maintain structural integrity of the characteristics of the presence of pepsin, sodium alginate and skim milk mixture as wall material of microencapsulation of Lactobacillus plantarum. Through the optimization design of the response surface, to determine the optimum process the conditions for extrusion of microcapsules: sodium alginate concentration 2.68%, calcium chloride concentration 0.20mol/L, microcapsule skim milk mass fraction of 4.17%. was prepared by the procedure of 3H in gastric juice, bacteria microcapsule in number can reach 8.79 * 109CFU/g.
2, on the basis of single factor test were 4 in the freeze-drying process of Lactobacillus plantarum better protective effect of cryoprotectant, the optimal compound cryoprotector formula by orthogonal test: 8% trehalose, lactose 8%, vitamin C0.2%, glycerol 3%. with the prescription of lyophilized microcapsules the cell survival rate reached 87.2%.
3, we use the improved two step vacuum drying method, namely vacuum constant temperature drying and vacuum freeze-drying, to dry the microcapsule. It can not only shorten the time of vacuum freeze-drying, but also increase the viability of viable cells in lyophilized capsules to 90.3%..
4, according to the optimized process, the survival rate of viable Lactobacillus microcapsules in the artificial gastric juice after treatment with 3H is about 80%, indicating that after microencapsulation, the resistance of probiotics to gastric acid environment is enhanced.
In 5, 45min in the artificial intestinal juice with Lactobacillus capsule preparation process optimized solution is obtained, the transmittance decreased to the minimum value, and not to the solid particles, indicating that the micro capsule has been basically complete disintegration, People's Republic of China reached the 2005 edition of the Pharmacopoeia standards. It can be judged, in accordance with the optimum process of Lactobacillus plantarum preparation of microcapsules with enteric coated well.
6, the freeze-dried 60d capsule storage at -20 deg.c, microcapsule cell survival rate close to 70%. storage 60d under the condition of 2 DEG C, there are still nearly 60% survival rate. So the optimized microencapsulation process obtained can effectively improve the storage stability of Lactobacillus plantarum in low temperature.

【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R371

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