BCR组库分析乙肝疫苗接种反应及初步机理分析

发布时间：2018-01-18 02:25

  本文关键词：BCR组库分析乙肝疫苗接种反应及初步机理分析　出处：《免疫学杂志》2017年09期 　论文类型：期刊论文

  更多相关文章： B细胞受体 免疫组库 乙肝疫苗 高通量测序

【摘要】：目的接种乙肝疫苗是控制乙肝流行最经济有效的方法,通过对3次乙肝注射疫苗前后的B细胞库进行高通量测序分析,探讨注射乙肝疫苗后体液免疫的动态过程。方法将志愿者接种乙肝疫苗前后的外周血样进行密度梯度离心获得淋巴细胞并提取RNA,逆转录为cDNA后对B细胞受体H链的CDR3区序列进行高通量测序,以揭示注射乙肝疫苗前后B细胞应答的变化,分析人外周血B细胞受体CDR3的多样性以及序列变化与疫苗应答之间的关系。结果志愿者外周血B细胞受体CDR3的种类和数量在接种疫苗后都有所降低,并表现出其特有的免疫学特征,包括高频取用的V、D、J家族、V-J家族配对发生了改变,CDR3区氨基酸的取用和长度分布也发生显著变化;并且接种疫苗后,志愿者外周血中存在某些寡克隆异常增殖的情况。结论大部分志愿者在接种疫苗后,B细胞受体组库的多样性等发生特征性改变,并且出现某些疫苗接种相关的寡克隆。B细胞受体高通量测序技术可以从克隆水平上监控乙肝疫苗接种后抗体产生和表达的动态变化过程。
[Abstract]:Objective inoculation of hepatitis B vaccine is the most economical and effective method to control the epidemic of hepatitis B. High-throughput sequencing analysis was carried out on the B cell bank before and after three times of hepatitis B vaccine injection. Objective: to investigate the dynamic process of humoral immunity after hepatitis B vaccine injection. Methods Peripheral blood samples from volunteers before and after hepatitis B vaccine were centrifuged with density gradient centrifugation to obtain lymphocytes and extract RNA. In order to reveal the changes of B cell response before and after injection of hepatitis B vaccine, high-throughput sequencing of the CDR3 region of B cell receptor H chain was carried out after reverse transcription into cDNA. The diversity of B cell receptor CDR3 in human peripheral blood and the relationship between the sequence change and vaccine response were analyzed. Results the type and number of B cell receptor CDR3 in peripheral blood of volunteers were decreased after vaccination. . It also showed its unique immunological characteristics, including the changes in the amino acid uptake and length distribution of the CDR3 domain. And after vaccination, there were abnormal proliferation of some oligoclonal clones in peripheral blood of volunteers. Conclusion the diversity of B cell receptor group library in most volunteers after inoculation has some characteristic changes. Moreover, some vaccine-related oligoclonal. B cell receptor high-throughput sequencing techniques can monitor the dynamic process of antibody production and expression after hepatitis B vaccine inoculation at clone level.
【作者单位】： 第三军医大学全军免疫学研究所;第三军医大学卫生处;
【基金】：国家自然科学基金国际(地区)合作与交流项目(81220108024)
【分类号】：R392-33
【正文快照】： 乙肝(viral hepatitis B)是由HBV引起的病毒性肝炎,防治乙肝仍是个世界性的问题[1]。在乙肝病毒感染预防中,接种疫苗是最安全有效的措施。疫苗接种后,可以刺激机体的免疫系统产生获得性免疫并形成免疫记忆,从而在较长时间内,甚至终生对其(81220108024)产生免疫[1-2]。获得性免，

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