丹酚酸A改善ZDF大鼠视网膜病变及其作用机制

发布时间：2018-01-19 02:06

本文关键词： 型糖尿病视网膜病变 Lp-PLA ZDF大鼠　出处：《中国比较医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察丹酚酸A(SAA)对Zucker糖尿病肥胖(ZDF)大鼠视网膜病变的干预作用,探讨SAA可能的作用机制。方法取7~8周龄雄性ZDF大鼠32只,Purina#5008饲料饲喂4周后,按血糖平均分为模型对照组,SAA高、低剂量组与二甲双胍(Met)组,每组8只。另取同龄雄性ZL大鼠8只作为正常对照。SAA低、高剂量组每日尾静脉注射SAA 0.5 mg/kg、1 mg/kg,Met组每日经口灌胃Met 200 mg/kg,连续给药12周,观察ZDF大鼠白内障发生率和视网膜病理学改变,并检测血糖血脂、糖化血红蛋白(Hb A1c)和血清白介素1(IL-1)、IL-6、氧化性低密度脂蛋白(ox-LDL)、丙二醛(MDA)含量和脂蛋白相关磷脂酶A2(Lp-PLA2)的活性,以及尿液中转铁蛋白(TRF)、视黄醇结合蛋白(RBP)的含量。结果模型对照组大鼠血糖、血脂及Hb A1c均上升,糖尿病性白内障发病率68.75%,视网膜基底增厚和微血管病变,血清IL-1、IL-6、ox-LDL和MDA含量和Lp-PLA2活性显著升高,尿液中TRF和RBP含量亦显著升高。给予SAA后,ZDF大鼠白内障发病率降低,视网膜病理学形态有不同程度的改善,血清IL-1、IL-6、ox-LDL、MDA含量和Lp-PLA2活性下降,尿液中TRF、RBP含量亦下降。结论 SAA能改善ZDF大鼠糖尿病视网膜病变,降低白内障发生率,其作用途径可能与抑制慢性炎症、防止脂质过氧化和降低Lp-PLA2活性有关。
[Abstract]:Objective to observe the effect of Salvianolic acid (SAA) on retinopathy in Zucker diabetic obese rats. To explore the possible mechanism of SAA. Methods 32 male ZDF rats aged 7 to 8 weeks were fed with the feed of Purina #5008 for 4 weeks, and were divided into model control group according to blood glucose. Low dose group and metformin metformin group, 8 rats in each group. Another 8 male ZL rats of the same age were taken as normal control. SAA was low, and SAA 0.5 mg/kg was injected daily in high dose group. One mg / kg Met group was given orally Met 200mg / kg daily for 12 weeks to observe the incidence of cataract and pathological changes of retina in ZDF rats. Blood glucose, blood lipids, glycosylated hemoglobin (HbA1c), interleukin-1 (IL-1) and interleukin-6 (IL-6), oxidizing low density lipoprotein (ox-LDL) were measured. The content of malondialdehyde (MDA) and the activity of lipoprotein associated phospholipase A _ 2 (Lp-PLA _ 2), as well as the urine transferrin (TRFs). Results the levels of blood glucose, blood lipid and HB A1c in the model control group were increased, and the incidence of diabetic cataract was 68.75%. The level of IL-6 ox-LDL and MDA and the activity of Lp-PLA2 were significantly increased in patients with retinal basement thickening and microangiopathy. The contents of TRF and RBP in urine were also significantly increased. The incidence of cataract was decreased and the pathological morphology of retina was improved in different degree. The content of Lp-PLA2 and the content of Lp-PLA2 in urine decreased. Conclusion SAA can improve diabetic retinopathy in ZDF rats. Reducing the incidence of cataract may be related to the inhibition of chronic inflammation, the prevention of lipid peroxidation and the reduction of Lp-PLA2 activity.
【作者单位】：浙江中医药大学动物实验研究中心/比较医学研究所;温州医科大学附属第二医院;
【基金】：浙江省中医药管理局重点项目(2015ZZ009) 浙江中医药大学比较医学创新团队(XTD201301)
【分类号】：R285.5;R-332
【正文快照】： 2.温州医科大学附属第二医院,浙江温州325027)and retinal pathology was observed,and levels of GLU,TC and Hb A1cin blood,transferrin(TRF)and retinol bindingprotein(RBP)in urine and levels of interleukin-1(IL-1),interleukin-6(IL-6),oxidized low density lipopr

【相似文献】