不同麻醉方式下经皮针刺旋切制备椎间盘退变模型

发布时间：2018-01-19 05:34

本文关键词： 脊柱椎间盘模型动物麻醉针刺组织构建组织工程脊柱退变体外模型动物模型兔微创　出处：《中国组织工程研究》2017年24期 　论文类型：期刊论文

【摘要】：背景:研究发现,不同的麻醉方式对大鼠的平均动脉压、收缩压和舒张压、动脉血p H值以及血液黏度均有明显的影响,但当前针对采用何种麻醉方法进行制备动物模型并未达成一致。目的:比较采用局部麻醉及全身麻醉方式下经皮针刺旋切制备的兔椎间盘退变模型的有效性。方法:将48只新西兰大白兔按随机分为局麻组和全麻组,局麻组采用0.5%利多卡因麻醉,全麻组动物予以腹腔注射体积分数3%戊巴比妥钠(30 mg/kg),2组均采用经皮微创针刺旋切兔L4/5、L5/6椎间盘建立椎间盘退变模型。观察2组动物的造模时间,并于造模后4,8,12,16周通过大体观察及MRI检测、组织病理学检查评价椎间盘退变情况。结果与结论:(1)大体观察:造模后2组髓核组织颜色逐渐暗且弹性降低;(2)MRI显示T2加权像椎间盘信号强度早期均未见明显改变,但信号强度随时间延长呈减弱趋势;(3)根据Pfirrmann分级法评价椎间盘退变程度:2组动物椎间盘退变程度均随时间延长逐渐加重(P0.05),2组术后各时间点椎间盘退变程度差异无显著性意义(P0.05);(4)Masson染色显示:造模后8,12周2组纤维环均出现不同程度排列不规整,但结构仍完整,16周后2组纤维环排列紊乱,甚至出现断裂现象,2组间无明显差异;(5)番红O染色示:2组造模后4周髓核细胞均未见明显减少,造模后16周2组髓核细胞均明显减少;(6)造模时间:局麻组平均(15.24±2.67)min明显要短于全麻组(25.64±6.85)min(P0.05);(7)结果说明,采用局麻和全麻方法经皮微创针刺旋切椎间盘均可成功建立椎间盘退变模型,但是在微创制备兔椎间盘退变模型中采用局麻具有操作时间更短、操作更简单的优点,且其造模效果与采用全身麻醉几乎相同。
[Abstract]:Background: it was found that different anesthetic methods had significant effects on mean arterial pressure, systolic and diastolic blood pressure, arterial blood pH and blood viscosity in rats. However, there is no agreement on the anesthetic methods to be used in the preparation of animal models. Methods: 48 New Zealand rabbits were randomly divided into local anesthesia group and general anesthesia group. The local anesthesia group was anesthetized with 0.5% lidocaine, and the general anesthesia group was given intraperitoneal injection of 3% pentobarbital sodium at 30 mg / kg. Both groups were treated with percutaneous minimally invasive acupuncture for L 4 / 5. The intervertebral disc degeneration model was established with L5 / 6 intervertebral disc. The model time of the two groups was observed, and the gross observation and MRI detection were carried out at the 4th week after the model. Results and conclusion: the color and elasticity of nucleus pulposus in the two groups decreased gradually after modeling. MRI showed that the signal intensity of T2-weighted images did not change obviously at the early stage, but the signal intensity decreased with time. (3) the degree of disc degeneration was evaluated by Pfirrmann grading method in both groups (P0.05). There was no significant difference in the degree of intervertebral disc degeneration between the two groups at different time points after operation (P 0.05). Masson staining showed that the two groups had irregular arrangement of fiber rings in different degrees at 812 weeks after modeling, but the structure was still intact. After 16 weeks, the arrangement of fiber rings in the two groups was disordered, and even the phenomenon of fracture appeared. There was no significant difference between the two groups. (5) Phanerin O staining showed that the number of nucleus pulposus cells did not decrease significantly at 4 weeks after modeling in group 2, but decreased significantly in group 2 at 16 weeks after modeling. (6) Modeling time: the average time of local anesthesia group was 15.24 卤2.67 minutes, which was significantly shorter than that of general anesthesia group (25.64 卤6.85 min, P 0.05). The results show that the model of intervertebral disc degeneration can be established successfully by local anesthesia and general anesthesia. However, local anesthesia has the advantages of shorter operation time and simpler operation in the minimally invasive model of rabbit intervertebral disc degeneration, and its effect is almost the same as that of general anesthesia.
【作者单位】：深圳市中医院骨三科;广州中医药大学第四临床医学院;
【基金】：深圳市科技计划项目(JCYJ20150401163247232) 深圳市卫生计生系统科研项目(201401066) 广东省科技厅(自筹项目)(2015[110号]) 广东省中医药局项目(20162126)~~
【分类号】：R-332;R681.5
【正文快照】： 文章快速阅读:比较局部麻醉及全身麻醉经皮针刺旋切制备兔椎间盘退变模型的有效性局麻组结果说明:大体观察及MRI检测、建立椎间盘采用局麻和全麻方法经皮微创针刺旋切组织病理学检查评价退变模型椎间盘均可成功建立椎间盘退变模型,椎间盘退变情况全麻组但局麻操作时间更短、操

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